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. 1993 Sep;61(9):3769–3774. doi: 10.1128/iai.61.9.3769-3774.1993

Natural killer cells do not play a dominant role in CD4+ subset differentiation in Candida albicans-infected mice.

L Romani 1, A Mencacci 1, E Cenci 1, R Spaccapelo 1, E Schiaffella 1, L Tonnetti 1, P Puccetti 1, F Bistoni 1
PMCID: PMC281076  PMID: 8359898

Abstract

The effects of in vivo administration of monoclonal antibodies against NK-1.1-bearing cells on the early production of gamma interferon (IFN-gamma) in vitro and development of Th1-associated immunity were studied in mice infected with a live vaccine strain of Candida albicans. At 1 and 4 days postinfection, natural killer (NK) cell-enriched fractions from the spleens of antibody-treated mice displayed a dramatic reduction in 5E6+ lymphocytes and negligible anti-YAC-1 cytotoxic activity in vitro. Nevertheless, the frequency of IFN-gamma-producing cells in those fractions was reduced by less than half, on average, by anti-NK-1.1 treatment in vivo. In addition, the antibody-treated and infected mice demonstrated unchanged T helper cell responses, as measured by yeast-specific footpad reactions, resistance to reinfection, occurrence of antibodies of different isotypes, and production in vitro of interleukin-2 (IL-2), IFN-gamma, IL-4, and IL-10 by CD4+ cells. Therefore, although NK cells may contribute to early IFN-gamma production in Candida-vaccinated mice, these cells apparently do not play a dominant role in the qualitative development of yeast-specific T helper responses.

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Selected References

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