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. 1993 Sep;61(9):3922–3927. doi: 10.1128/iai.61.9.3922-3927.1993

Purification and partial characterization of a product from Yersinia pseudotuberculosis with the ability to activate human T cells.

T Miyoshi-Akiyama 1, K Imanishi 1, T Uchiyama 1
PMCID: PMC281095  PMID: 8359914

Abstract

We tried to purify a substance exhibiting mitogenicity for human peripheral blood lymphocytes from Yersinia pseudotuberculosis isolated from patients with Y. pseudotuberculosis infection manifesting acute and systemic clinical symptoms. The supernatant of a suspension of the bacteria disrupted by sonication was serially chromatographed on DEAE-Sepharose fast-flow, Sephacryl S-100 HR, and TSK-gel G2000SW high-pressure liquid chromatography columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that the purified mitogenically active substance migrated as a single band corresponding to a molecular mass of 21 kDa. We designated the purified substance Y. pseudotuberculosis-derived mitogen (YPM). YPM stimulated human peripheral T cells to proliferate and produce interleukin-2 at 0.1 ng/ml or more. YPM-induced T-cell activation required the expression of HLA class II molecules on accessory cells.

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Selected References

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