Abstract
Selective attachment of indigenous, apathogenic, segmented filamentous bacteria to follicle-associated epithelia of terminal ileal Peyer's patches involves both the specialized M cells and normal enterocytes. A striking, highly localized cytoplasmic accumulation of polymerized actin, which resembles that associated with adherence of enteropathogenic Escherichia coli to cultured epithelial cells, occurs at attachment sites of segmented filamentous bacteria. The epithelial response to the attachment of microorganisms may thus involve mechanisms which are the same for diverse microorganisms.
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Selected References
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- Baldwin T. J., Lee-Delaunay M. B., Knutton S., Williams P. H. Calcium-calmodulin dependence of actin accretion and lethality in cultured HEp-2 cells infected with enteropathogenic Escherichia coli. Infect Immun. 1993 Feb;61(2):760–763. doi: 10.1128/iai.61.2.760-763.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baldwin T. J., Ward W., Aitken A., Knutton S., Williams P. H. Elevation of intracellular free calcium levels in HEp-2 cells infected with enteropathogenic Escherichia coli. Infect Immun. 1991 May;59(5):1599–1604. doi: 10.1128/iai.59.5.1599-1604.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase D. G., Erlandsen S. L. Evidence for a complex life cycle and endospore formation in the attached, filamentous, segmented bacterium from murine ileum. J Bacteriol. 1976 Jul;127(1):572–583. doi: 10.1128/jb.127.1.572-583.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis C. P., Savage D. C. Habitat, succession, attachment, and morphology of segmented, filamentous microbes indigenous to the murine gastrointestinal tract. Infect Immun. 1974 Oct;10(4):948–956. doi: 10.1128/iai.10.4.948-956.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donnenberg M. S., Calderwood S. B., Donohue-Rolfe A., Keusch G. T., Kaper J. B. Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells. Infect Immun. 1990 Jun;58(6):1565–1571. doi: 10.1128/iai.58.6.1565-1571.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erlandsen S. L., Chase D. G. Morphological alterations in the microvillous border of villous epithelial cells produced by intestinal microorganisms. Am J Clin Nutr. 1974 Nov;27(11):1277–1286. doi: 10.1093/ajcn/27.11.1277. [DOI] [PubMed] [Google Scholar]
- Finlay B. B., Rosenshine I., Donnenberg M. S., Kaper J. B. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect Immun. 1992 Jun;60(6):2541–2543. doi: 10.1128/iai.60.6.2541-2543.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finlay B. B., Ruschkowski S., Dedhar S. Cytoskeletal rearrangements accompanying salmonella entry into epithelial cells. J Cell Sci. 1991 Jun;99(Pt 2):283–296. doi: 10.1242/jcs.99.2.283. [DOI] [PubMed] [Google Scholar]
- Hampton J. C., Rosario B. The attachment of microorganisms to epithelial cells in the distal ileum of the mouse. Lab Invest. 1965 Aug;14(8):1464–1481. [PubMed] [Google Scholar]
- Jepson M. A., Simmons N. L., Savidge T. C., James P. S., Hirst B. H. Selective binding and transcytosis of latex microspheres by rabbit intestinal M cells. Cell Tissue Res. 1993 Mar;271(3):399–405. doi: 10.1007/BF02913722. [DOI] [PubMed] [Google Scholar]
- Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839–7843. doi: 10.1073/pnas.87.20.7839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klaasen H. L., Koopman J. P., Poelma F. G., Beynen A. C. Intestinal, segmented, filamentous bacteria. FEMS Microbiol Rev. 1992 Jun;8(3-4):165–180. doi: 10.1111/j.1574-6968.1992.tb04986.x. [DOI] [PubMed] [Google Scholar]
- Klaasen H. L., Van der Heijden P. J., Stok W., Poelma F. G., Koopman J. P., Van den Brink M. E., Bakker M. H., Eling W. M., Beynen A. C. Apathogenic, intestinal, segmented, filamentous bacteria stimulate the mucosal immune system of mice. Infect Immun. 1993 Jan;61(1):303–306. doi: 10.1128/iai.61.1.303-306.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knutton S., Baldwin T., Williams P. H., McNeish A. S. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun. 1989 Apr;57(4):1290–1298. doi: 10.1128/iai.57.4.1290-1298.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knutton S., Lloyd D. R., McNeish A. S. Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun. 1987 Jan;55(1):69–77. doi: 10.1128/iai.55.1.69-77.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koopman J. P., Stadhouders A. M., Kennis H. M., De Boer H. The attachment of filamentous segmented micro-organisms to the distal ileum wall of the mouse: a scanning and transmission electron microscopy study. Lab Anim. 1987 Jan;21(1):48–52. doi: 10.1258/002367787780740743. [DOI] [PubMed] [Google Scholar]
- Kraehenbuhl J. P., Neutra M. R. Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev. 1992 Oct;72(4):853–879. doi: 10.1152/physrev.1992.72.4.853. [DOI] [PubMed] [Google Scholar]
- Rosenshine I., Donnenberg M. S., Kaper J. B., Finlay B. B. Signal transduction between enteropathogenic Escherichia coli (EPEC) and epithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangement and bacterial uptake. EMBO J. 1992 Oct;11(10):3551–3560. doi: 10.1002/j.1460-2075.1992.tb05438.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Savage D. C. Localization of certain indigenous microorganisms on the ileal villi of rats. J Bacteriol. 1969 Mar;97(3):1505–1506. doi: 10.1128/jb.97.3.1505-1506.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snellen J. E., Savage D. C. Freeze-fracture study of the filamentous, segmented microorganism attached to the murine small bowel. J Bacteriol. 1978 Jun;134(3):1099–1107. doi: 10.1128/jb.134.3.1099-1107.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]