Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Sep;61(9):4005–4007. doi: 10.1128/iai.61.9.4005-4007.1993

Immunological properties of ribosomal proteins from Mycobacterium bovis BCG.

S Tantimavanich 1, S Nagai 1, H Nomaguchi 1, M Kinomoto 1, N Ohara 1, T Yamada 1
PMCID: PMC281109  PMID: 8359926

Abstract

Two proteins with molecular mass 65 kDa, a heat shock protein, and an S1-like protein were found in a 30S ribosomal subunit from Mycobacterium bovis BCG. The 17-kDa protein in the 30S subunit was homologous to alpha-crystallin heat shock protein, and the 16-kDa protein in the 50S subunit was homologous to the L7/L12 protein. The latter provoked a strong delayed-type hypersensitivity reaction in the sensitized guinea pigs. The GroES-like protein (12 kDa) loosely associated with ribosomes.

Full text

PDF
4005

Images in this article

4006Image
on p.4006
4006Image
on p.4006

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baird P. N., Hall L. M., Coates A. R. A major antigen from Mycobacterium tuberculosis which is homologous to the heat shock proteins groES from E. coli and the htpA gene product of Coxiella burneti. Nucleic Acids Res. 1988 Sep 26;16(18):9047–9047. doi: 10.1093/nar/16.18.9047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker R. E., Hill W. E., Larson C. L. Delayed hypersensitivity reactions provoked by ribosomes from acid-fast bacilli. I. Ribosomal isolation, characterization, delayed hypersensitivity, and specificity. Infect Immun. 1972 Sep;6(3):258–265. doi: 10.1128/iai.6.3.258-265.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker R. E., Hill W. E., Larson C. L. Ribosomes of acid-fast bacilli: immunogenicity, serology, and in vitro correlates of delayed hypersensitivity. Infect Immun. 1973 Aug;8(2):236–244. doi: 10.1128/iai.8.2.236-244.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gregory R. L. Microbial ribosomal vaccines. Rev Infect Dis. 1986 Mar-Apr;8(2):208–217. doi: 10.1093/clinids/8.2.208. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Loge R. V., Hill W. E., Baker R. E., Larson C. L. Delayed hypersensitivity reactions provoked by ribosomes from acid-fast bacilli: physical characteristics and immunological aspects of core ribosomal proteins from Mycobacterium smegmatis. Infect Immun. 1974 Mar;9(3):489–496. doi: 10.1128/iai.9.3.489-496.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed] [Google Scholar]
  8. Minden P., Kelleher P. J., Freed J. H., Nielsen L. D., Brennan P. J., McPheron L., McClatchy J. K. Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG. Infect Immun. 1984 Nov;46(2):519–525. doi: 10.1128/iai.46.2.519-525.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miura K., Nagai S., Kinomoto M., Haga S., Tokunaga T. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70. Infect Immun. 1983 Feb;39(2):540–545. doi: 10.1128/iai.39.2.540-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nagai S., Matsumoto J., Nagasuga T. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG. Infect Immun. 1981 Mar;31(3):1152–1160. doi: 10.1128/iai.31.3.1152-1160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagai S., Wiker H. G., Harboe M., Kinomoto M. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect Immun. 1991 Jan;59(1):372–382. doi: 10.1128/iai.59.1.372-382.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nomaguchi H., Matsuoka M., Kohsaka K., Nakata A., Ito T. Overproduction, affinity purification and characterization of 65-kDa protein of Mycobacterium leprae in Escherichia coli. Int J Lepr Other Mycobact Dis. 1989 Dec;57(4):817–824. [PubMed] [Google Scholar]
  13. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  14. Ortiz-Ortiz L., Solarolo E. B., Bojalil L. F. Delayed hypersensitivity to ribosomal protein from BCG. J Immunol. 1971 Oct;107(4):1022–1026. [PubMed] [Google Scholar]
  15. Verbon A., Hartskeerl R. A., Schuitema A., Kolk A. H., Young D. B., Lathigra R. The 14,000-molecular-weight antigen of Mycobacterium tuberculosis is related to the alpha-crystallin family of low-molecular-weight heat shock proteins. J Bacteriol. 1992 Feb;174(4):1352–1359. doi: 10.1128/jb.174.4.1352-1359.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamada T. Characterization of an S1-like protein in Mycobacterium smegmatis ribosomes. FEBS Lett. 1982 Jun 7;142(2):267–270. doi: 10.1016/0014-5793(82)80149-x. [DOI] [PubMed] [Google Scholar]
  18. Yamada T., Mizugichi Y., Nierhaus K. H., Wittmann H. G. Resistance to viomycin conferred by RNA of either ribosomal subunit. Nature. 1978 Oct 5;275(5679):460–461. doi: 10.1038/275460a0. [DOI] [PubMed] [Google Scholar]
  19. Yamaguchi R., Matsuo K., Yamazaki A., Nagai S., Terasaka K., Yamada T. Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression. FEBS Lett. 1988 Nov 21;240(1-2):115–117. doi: 10.1016/0014-5793(88)80350-8. [DOI] [PubMed] [Google Scholar]
  20. Young D. B., Garbe T. R. Heat shock proteins and antigens of Mycobacterium tuberculosis. Infect Immun. 1991 Sep;59(9):3086–3093. doi: 10.1128/iai.59.9.3086-3093.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES