Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Sep;61(9):4017–4020. doi: 10.1128/iai.61.9.4017-4020.1993

Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae.

R S Munson Jr 1, S Grass 1, R West 1
PMCID: PMC281112  PMID: 8359929

Abstract

The gene for outer membrane protein P5 of Haemophilus influenzae was identified by immunological screening of a genomic lambda EMBL3 library of the serotype b strain 1613. The gene was subcloned, and plasmid clones expressing P5 were identified by immunologic screening. The gene for outer membrane protein P5 was sequenced. The mature protein has a molecular weight of 35,628. The protein is 50% identical and 65% similar to the OmpA protein of Escherichia coli.

Full text

PDF
4017

Images in this article

4017Image
on p.4017

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake M. S., Wetzler L. M., Gotschlich E. C., Rice P. A. Protein III: structure, function, and genetics. Clin Microbiol Rev. 1989 Apr;2 (Suppl):S60–S63. doi: 10.1128/cmr.2.suppl.s60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulton J. W., Wan D. T. The outer membrane of haemophilus influenzae type b: cell envelope associations of major proteins. Can J Microbiol. 1983 Feb;29(2):280–287. doi: 10.1139/m83-046. [DOI] [PubMed] [Google Scholar]
  3. Deich R. A., Metcalf B. J., Finn C. W., Farley J. E., Green B. A. Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae. J Bacteriol. 1988 Feb;170(2):489–498. doi: 10.1128/jb.170.2.489-498.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Finnen R. L., Martin N. L., Siehnel R. J., Woodruff W. A., Rosok M., Hancock R. E. Analysis of the Pseudomonas aeruginosa major outer membrane protein OprF by use of truncated OprF derivatives and monoclonal antibodies. J Bacteriol. 1992 Aug;174(15):4977–4985. doi: 10.1128/jb.174.15.4977-4985.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hughes E. E., Gilleland L. B., Gilleland H. E., Jr Synthetic peptides representing epitopes of outer membrane protein F of Pseudomonas aeruginosa that elicit antibodies reactive with whole cells of heterologous immunotype strains of P. aeruginosa. Infect Immun. 1992 Sep;60(9):3497–3503. doi: 10.1128/iai.60.9.3497-3503.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klugman K. P., Gotschlich E. C., Blake M. S. Sequence of the structural gene (rmpM) for the class 4 outer membrane protein of Neisseria meningitidis, homology of the protein to gonococcal protein III and Escherichia coli OmpA, and construction of meningococcal strains that lack class 4 protein. Infect Immun. 1989 Jul;57(7):2066–2071. doi: 10.1128/iai.57.7.2066-2071.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  9. Morona R., Klose M., Henning U. Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins. J Bacteriol. 1984 Aug;159(2):570–578. doi: 10.1128/jb.159.2.570-578.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Munson R. S., Jr, Granoff D. M. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect Immun. 1985 Sep;49(3):544–549. doi: 10.1128/iai.49.3.544-549.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Munson R., Jr, Grass S., Einhorn M., Bailey C., Newell C. Comparative analysis of the structures of the outer membrane protein P1 genes from major clones of Haemophilus influenzae type b. Infect Immun. 1989 Nov;57(11):3300–3305. doi: 10.1128/iai.57.11.3300-3305.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Munson R., Jr, Grass S. Purification, cloning, and sequence of outer membrane protein P1 of Haemophilus influenzae type b. Infect Immun. 1988 Sep;56(9):2235–2242. doi: 10.1128/iai.56.9.2235-2242.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nikaido H., Nikaido K., Harayama S. Identification and characterization of porins in Pseudomonas aeruginosa. J Biol Chem. 1991 Jan 15;266(2):770–779. [PubMed] [Google Scholar]
  14. Puohiniemi R., Karvonen M., Vuopio-Varkila J., Muotiala A., Helander I. M., Sarvas M. A strong antibody response to the periplasmic C-terminal domain of the OmpA protein of Escherichia coli is produced by immunization with purified OmpA or with whole E. coli or Salmonella typhimurium bacteria. Infect Immun. 1990 Jun;58(6):1691–1696. doi: 10.1128/iai.58.6.1691-1696.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rice P. A. Molecular basis for serum resistance in Neisseria gonorrhoeae. Clin Microbiol Rev. 1989 Apr;2 (Suppl):S112–S117. doi: 10.1128/cmr.2.suppl.s112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spinola S. M., Griffiths G. E., Shanks K. L., Blake M. S. The major outer membrane protein of Haemophilus ducreyi is a member of the OmpA family of proteins. Infect Immun. 1993 Apr;61(4):1346–1351. doi: 10.1128/iai.61.4.1346-1351.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sugawara E., Nikaido H. Pore-forming activity of OmpA protein of Escherichia coli. J Biol Chem. 1992 Feb 5;267(4):2507–2511. [PubMed] [Google Scholar]
  18. Weiser J. N., Gotschlich E. C. Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1. Infect Immun. 1991 Jul;59(7):2252–2258. doi: 10.1128/iai.59.7.2252-2258.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilson M. E. The heat-modifiable outer membrane protein of Actinobacillus actinomycetemcomitans: relationship to OmpA proteins. Infect Immun. 1991 Jul;59(7):2505–2507. doi: 10.1128/iai.59.7.2505-2507.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woodruff W. A., Hancock R. E. Pseudomonas aeruginosa outer membrane protein F: structural role and relationship to the Escherichia coli OmpA protein. J Bacteriol. 1989 Jun;171(6):3304–3309. doi: 10.1128/jb.171.6.3304-3309.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Alphen L., Riemens T., Poolman J., Zanen H. C. Characteristics of major outer membrane proteins of Haemophilus influenzae. J Bacteriol. 1983 Aug;155(2):878–885. doi: 10.1128/jb.155.2.878-885.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES