Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Oct;61(10):4027–4032. doi: 10.1128/iai.61.10.4027-4032.1993

Antibody-mediated enhancement of Legionella pneumophila-induced interleukin 1 activity.

R H Widen 1, C A Newton 1, T W Klein 1, H Friedman 1
PMCID: PMC281119  PMID: 8406789

Abstract

The ability of antibody specific for Legionella pneumophila to enhance the induction of interleukin 1 (IL-1) production by murine peritoneal, splenic, and pulmonary macrophages in response to the bacterium was examined. Two preparations of L. pneumophila were utilized, a formalin-killed whole-cell preparation and viable bacteria. We measured both secreted (sIL-1) and cell membrane-associated (mIL-1) activities after incubation of the macrophages with the bacterial preparations in the presence or absence of the antibody. Both bacterial preparations induced sIL-1 and mIL-1 activities in each of the macrophage populations tested. These activities were generally enhanced by pretreating the bacteria with antibody, with the greatest enhancing activity observed for the formalin-killed preparations at lower doses of bacteria.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auron P. E., Webb A. C., Rosenwasser L. J., Mucci S. F., Rich A., Wolff S. M., Dinarello C. A. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7907–7911. doi: 10.1073/pnas.81.24.7907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bacle F., Haeffner-Cavaillon N., Laude M., Couturier C., Kazatchkine M. D. Induction of IL-1 release through stimulation of the C3b/C4b complement receptor type one (CR1, CD35) on human monocytes. J Immunol. 1990 Jan 1;144(1):147–152. [PubMed] [Google Scholar]
  3. Bakouche O., Moreau J. L., Lachman L. B. Acylation of cell-associated IL-1 by palmitic acid. J Immunol. 1991 Oct 1;147(7):2164–2169. [PubMed] [Google Scholar]
  4. Bennett B. Isolation and cultivation in vitro of macrophages from various sources in the mouse. Am J Pathol. 1966 Jan;48(1):165–181. [PMC free article] [PubMed] [Google Scholar]
  5. Beuscher H. U., Nickells M. W., Colten H. R. The precursor of interleukin-1 alpha is phosphorylated at residue serine 90. J Biol Chem. 1988 Mar 15;263(8):4023–4028. [PubMed] [Google Scholar]
  6. Blanchard D. K., Djeu J. Y., Klein T. W., Friedman H., Stewart W. E., 2nd Induction of tumor necrosis factor by Legionella pneumophila. Infect Immun. 1987 Feb;55(2):433–437. doi: 10.1128/iai.55.2.433-437.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Blanchard D. K., Friedman H., Stewart W. E., 2nd, Klein T. W., Djeu J. Y. Role of gamma interferon in induction of natural killer activity by Legionella pneumophila in vitro and in an experimental murine infection model. Infect Immun. 1988 May;56(5):1187–1193. doi: 10.1128/iai.56.5.1187-1193.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Blyden G., Handschumacher R. E. Purification and properties of human lymphocyte activating factor (LAF). J Immunol. 1977 May;118(5):1631–1638. [PubMed] [Google Scholar]
  9. Brody D. T., Durum S. K. Membrane IL-1: IL-1 alpha precursor binds to the plasma membrane via a lectin-like interaction. J Immunol. 1989 Aug 15;143(4):1183–1187. [PubMed] [Google Scholar]
  10. Chen L., Suzuki Y., Wheelock E. F. Interferon-gamma synergizes with tumor necrosis factor and with interleukin 1 and requires the presence of both monokines to induce antitumor cytotoxic activity in macrophages. J Immunol. 1987 Dec 15;139(12):4096–4101. [PubMed] [Google Scholar]
  11. Chou Y. K., Sherwood T., Virella G. Erythrocyte-bound immune complexes trigger the release of interleukin-1 from human monocytes. Cell Immunol. 1985 Mar;91(1):308–314. doi: 10.1016/0008-8749(85)90054-1. [DOI] [PubMed] [Google Scholar]
  12. Conlon P. J., Grabstein K. H., Alpert A., Prickett K. S., Hopp T. P., Gillis S. Localization of human mononuclear cell interleukin 1. J Immunol. 1987 Jul 1;139(1):98–102. [PubMed] [Google Scholar]
  13. Czuprynski C. J., Brown J. F., Young K. M., Cooley A. J., Kurtz R. S. Effects of murine recombinant interleukin 1 alpha on the host response to bacterial infection. J Immunol. 1988 Feb 1;140(3):962–968. [PubMed] [Google Scholar]
  14. Dinarello C. A. Interleukin-1 and interleukin-1 antagonism. Blood. 1991 Apr 15;77(8):1627–1652. [PubMed] [Google Scholar]
  15. Dinarello C. A. Role of interleukin-1 in infectious diseases. Immunol Rev. 1992 Jun;127:119–146. doi: 10.1111/j.1600-065x.1992.tb01411.x. [DOI] [PubMed] [Google Scholar]
  16. Dower S. K., Kronheim S. R., Hopp T. P., Cantrell M., Deeley M., Gillis S., Henney C. S., Urdal D. L. The cell surface receptors for interleukin-1 alpha and interleukin-1 beta are identical. Nature. 1986 Nov 20;324(6094):266–268. doi: 10.1038/324266a0. [DOI] [PubMed] [Google Scholar]
  17. Durum S. K., Schmidt J. A., Oppenheim J. J. Interleukin 1: an immunological perspective. Annu Rev Immunol. 1985;3:263–287. doi: 10.1146/annurev.iy.03.040185.001403. [DOI] [PubMed] [Google Scholar]
  18. England A. C., 3rd, Fraser D. W., Plikaytis B. D., Tsai T. F., Storch G., Broome C. V. Sporadic legionellosis in the United States: the first thousand cases. Ann Intern Med. 1981 Feb;94(2):164–170. doi: 10.7326/0003-4819-94-2-164. [DOI] [PubMed] [Google Scholar]
  19. Freedman A. P., Katz S. M. The prevalence of serum antibodies to Legionella pneumophila in patients with chronic pulmonary disease. Am Rev Respir Dis. 1981 Feb;123(2):238–239. doi: 10.1164/arrd.1981.123.2.238. [DOI] [PubMed] [Google Scholar]
  20. Friedman H., Widen R., Klein T., Searls L., Cabrian K. Legionella pneumophila-induced blastogenesis of murine lymphoid cells in vitro. Infect Immun. 1984 Jan;43(1):314–319. doi: 10.1128/iai.43.1.314-319.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Goodman M. G., Chenoweth D. E., Weigle W. O. Induction of interleukin 1 secretion and enhancement of humoral immunity by binding of human C5a to macrophage surface C5a receptors. J Exp Med. 1982 Sep 1;156(3):912–917. doi: 10.1084/jem.156.3.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Haley C. E., Cohen M. L., Halter J., Meyer R. D. Nosocomial Legionnaires' disease: a continuing common-source epidemic at Wadsworth Medical Center. Ann Intern Med. 1979 Apr;90(4):583–586. doi: 10.7326/0003-4819-90-4-583. [DOI] [PubMed] [Google Scholar]
  23. Horwitz M. A., Silverstein S. C. Activated human monocytes inhibit the intracellular multiplication of Legionnaires' disease bacteria. J Exp Med. 1981 Nov 1;154(5):1618–1635. doi: 10.1084/jem.154.5.1618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Horwitz M. A., Silverstein S. C. Interaction of the Legionnaires' disease bacterium (Legionella pneumophila) with human phagocytes. I. L. pneumophila resists killing by polymorphonuclear leukocytes, antibody, and complement. J Exp Med. 1981 Feb 1;153(2):386–397. doi: 10.1084/jem.153.2.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Horwitz M. A., Silverstein S. C. Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes. J Clin Invest. 1980 Sep;66(3):441–450. doi: 10.1172/JCI109874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Jirillo E., Decandia P., Ribaud M. R., Cannuscio B., De Simone C., Antonaci S. Enhancement of polymorphonuclear cell phagocytosis by lipid A-activated monocytes via cell-to-cell contact. A possible role for membrane-associated cytokines. Immunopharmacol Immunotoxicol. 1992;14(3):343–354. doi: 10.3109/08923979209005398. [DOI] [PubMed] [Google Scholar]
  27. Klein G. C., Jones W. L., Feeley J. C. Upper limit of normal titer for detection of antibodies to Legionella pneumophila by the microagglutination test. J Clin Microbiol. 1979 Nov;10(5):754–755. doi: 10.1128/jcm.10.5.754-755.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Klein T. W., Newton C. A., Blanchard D. K., Widen R., Friedman H. Induction of interleukin 1 by Legionella pneumophila antigens in mouse macrophage and human mononuclear leukocyte cultures. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jul;265(3-4):462–471. doi: 10.1016/s0176-6724(87)80265-1. [DOI] [PubMed] [Google Scholar]
  29. Kurt-Jones E. A., Beller D. I., Mizel S. B., Unanue E. R. Identification of a membrane-associated interleukin 1 in macrophages. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1204–1208. doi: 10.1073/pnas.82.4.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Kurt-Jones E. A., Fiers W., Pober J. S. Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts. J Immunol. 1987 Oct 1;139(7):2317–2324. [PubMed] [Google Scholar]
  31. Lomedico P. T., Gubler U., Hellmann C. P., Dukovich M., Giri J. G., Pan Y. C., Collier K., Semionow R., Chua A. O., Mizel S. B. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. 1984 Nov 29-Dec 5Nature. 312(5993):458–462. doi: 10.1038/312458a0. [DOI] [PubMed] [Google Scholar]
  32. Matsushima K., Taguchi M., Kovacs E. J., Young H. A., Oppenheim J. J. Intracellular localization of human monocyte associated interleukin 1 (IL 1) activity and release of biologically active IL 1 from monocytes by trypsin and plasmin. J Immunol. 1986 Apr 15;136(8):2883–2891. [PubMed] [Google Scholar]
  33. Minnich-Carruth L. L., Suttles J., Mizel S. B. Evidence against the existence of a membrane form of murine IL-1 alpha. J Immunol. 1989 Jan 15;142(2):526–530. [PubMed] [Google Scholar]
  34. Mizel S. B., Oppenheim J. J., Rosentreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by a macrophage cell line, P388D1. II. Biochemical characterization of LAF induced by activated T cells and LPS. J Immunol. 1978 May;120(5):1504–1508. [PubMed] [Google Scholar]
  35. Morgan E. L., Hobbs M. V., Noonan D. J., Weigle W. O. Induction of IL-1 secretion from human monocytes by Fc region subfragments of human IgG1. J Immunol. 1988 May 1;140(9):3014–3020. [PubMed] [Google Scholar]
  36. Suttles J., Carruth L. M., Mizel S. B. Detection of IL-1 alpha and IL-1 beta in the supernatants of paraformaldehyde-treated human monocytes. Evidence against a membrane form of IL-1. J Immunol. 1990 Jan 1;144(1):170–174. [PubMed] [Google Scholar]
  37. Vogel S. N., Kaufman E. N., Tate M. D., Neta R. Recombinant interleukin-1 alpha and recombinant tumor necrosis factor alpha synergize in vivo to induce early endotoxin tolerance and associated hematopoietic changes. Infect Immun. 1988 Oct;56(10):2650–2657. doi: 10.1128/iai.56.10.2650-2657.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Wilkinson H. W., Cruce D. D., Broome C. V. Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera. J Clin Microbiol. 1981 Jan;13(1):139–146. doi: 10.1128/jcm.13.1.139-146.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Winn W. C., Jr Legionnaires disease: historical perspective. Clin Microbiol Rev. 1988 Jan;1(1):60–81. doi: 10.1128/cmr.1.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Yamamoto Y., Klein T. W., Newton C. A., Widen R., Friedman H. Differential growth of Legionella pneumophila in guinea pig versus mouse macrophage cultures. Infect Immun. 1987 Jun;55(6):1369–1374. doi: 10.1128/iai.55.6.1369-1374.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES