FIGURE 6.

A model for AK028326 and AK141205 lncRNA placement into the Oct4 and Nanog regulatory network. (Black lines) Positive regulation; (blue lines) repression. Oct4 and Nanog are required for self-renewal, and Oct4 activates AK028326, while Nanog represses AK141205. Reduction of AK028326 exhibited a correlated reduction in Oct4 mRNA levels with a concordant up-regulation of Trophoblast lineage transcripts, though the mechanism is unclear. Overexpression of AK141205 led to up-regulation of Oct4 with a corresponding up-regulation of endodermal markers.