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. 1993 Oct;61(10):4375–4381. doi: 10.1128/iai.61.10.4375-4381.1993

Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.

S Kubo 1, H Kubota 1, Y Ohnishi 1, T Morita 1, T Matsuya 1, A Matsushiro 1
PMCID: PMC281169  PMID: 8406828

Abstract

We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in the shuttle vector pVA838. S. gordonii transformed with this plasmid (pMNK-4) expressed and secreted mature Arthrobacter dextranase. The transformant was found to repress the firm adherence of water-insoluble glucan in a coculture experiment with cariogenic bacteria, Streptococcus sobrinus, in the presence of sucrose. Such genetically engineered oral bacteria could provide a therapy to prevent dental caries.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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