Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Oct;61(10):4498–4500. doi: 10.1128/iai.61.10.4498-4500.1993

Isotypic analysis of Plasmodium falciparum-specific antibodies and their relation to protection in Madagascar.

B Dubois 1, P Deloron 1, P Astagneau 1, C Chougnet 1, J P Lepers 1
PMCID: PMC281187  PMID: 7691751

Abstract

We previously reported that antibodies to the central repeat (DDEHVEEPTVA) of Pf155/RESA, a major Plasmodium falciparum antigen, were negatively related to antimalarial protection. We measured levels of isotype antibodies to this epitope and to P. falciparum in 76 Madagascan subjects. Immunoglobulin G (IgG) and isotype antibodies specific for P. falciparum had similar levels in individuals who were considered protected and those who were not (as determined during a longitudinal follow-up). The levels of IgG1 antibodies to (DDEHVEEPTVA)3 were higher in nonprotected subjects. The levels of total IgG and of other isotype antibodies had a similar tendency to be higher in nonprotected than in protected individuals.

Full text

PDF
4498

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astagneau P., Lepers J. P., Chougnet C., Gaudebout C., Andriamangatiana-Rason M. D., Larouzé B., Deloron P. Assessment of the protective value of antibodies to the Plasmodium falciparum ring-infected erythrocyte surface antigen (RESA): an epidemiologic study in Madagascar. Am J Epidemiol. 1991 Jan 15;133(2):177–184. doi: 10.1093/oxfordjournals.aje.a115856. [DOI] [PubMed] [Google Scholar]
  2. Berzins K., Perlmann H., Wåhlin B., Carlsson J., Wahlgren M., Udomsangpetch R., Björkman A., Patarroyo M. E., Perlmann P. Rabbit and human antibodies to a repeated amino acid sequence of a Plasmodium falciparum antigen, Pf 155, react with the native protein and inhibit merozoite invasion. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1065–1069. doi: 10.1073/pnas.83.4.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouharoun-Tayoun H., Druilhe P. Plasmodium falciparum malaria: evidence for an isotype imbalance which may be responsible for delayed acquisition of protective immunity. Infect Immun. 1992 Apr;60(4):1473–1481. doi: 10.1128/iai.60.4.1473-1481.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chizzolini C., Delaporte E., Kaufmann M. H., Akue J. P., Verdini A. S., Pessi A., del Giudice G. Age-related prevalence of antibody response against three different, defined Plasmodium falciparum antigens in children from the Haut-Ogooué province in Gabon. Trans R Soc Trop Med Hyg. 1989 Mar-Apr;83(2):147–151. doi: 10.1016/0035-9203(89)90619-6. [DOI] [PubMed] [Google Scholar]
  5. Coppel R. L., Cowman A. F., Anders R. F., Bianco A. E., Saint R. B., Lingelbach K. R., Kemp D. J., Brown G. V. Immune sera recognize on erythrocytes Plasmodium falciparum antigen composed of repeated amino acid sequences. 1984 Aug 30-Sep 5Nature. 310(5980):789–792. doi: 10.1038/310789a0. [DOI] [PubMed] [Google Scholar]
  6. Deloron P., Campbell G. H., Brandling-Bennett D., Roberts J. M., Schwartz I. K., Odera J. S., Lal A. A., Osanga C. O., de la Cruz V., McCutchan T. M. Antibodies to Plasmodium falciparum ring-infected erythrocyte surface antigen and P. falciparum and P. malariae circumsporozoite proteins: seasonal prevalence in Kenyan villages. Am J Trop Med Hyg. 1989 Oct;41(4):395–399. doi: 10.4269/ajtmh.1989.41.395. [DOI] [PubMed] [Google Scholar]
  7. Deloron P., Cot M. Antibodies to the ring-infected erythrocyte surface antigen and the circumsporozoite protein of Plasmodium falciparum in a rural community from Burkina Faso. Trans R Soc Trop Med Hyg. 1990 Mar-Apr;84(2):191–195. doi: 10.1016/0035-9203(90)90250-i. [DOI] [PubMed] [Google Scholar]
  8. Khalife J., Dunne D. W., Richardson B. A., Mazza G., Thorne K. J., Capron A., Butterworth A. E. Functional role of human IgG subclasses in eosinophil-mediated killing of schistosomula of Schistosoma mansoni. J Immunol. 1989 Jun 15;142(12):4422–4427. [PubMed] [Google Scholar]
  9. Lepers J. P., Deloron P., Fontenille D., Coulanges P. Reappearance of falciparum malaria in central highland plateaux of Madagascar. Lancet. 1988 Mar 12;1(8585):586–586. doi: 10.1016/s0140-6736(88)91375-x. [DOI] [PubMed] [Google Scholar]
  10. Marsh K., Otoo L., Hayes R. J., Carson D. C., Greenwood B. M. Antibodies to blood stage antigens of Plasmodium falciparum in rural Gambians and their relation to protection against infection. Trans R Soc Trop Med Hyg. 1989 May-Jun;83(3):293–303. doi: 10.1016/0035-9203(89)90478-1. [DOI] [PubMed] [Google Scholar]
  11. Perlmann H., Berzins K., Wahlgren M., Carlsson J., Björkman A., Patarroyo M. E., Perlmann P. Antibodies in malarial sera to parasite antigens in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J Exp Med. 1984 Jun 1;159(6):1686–1704. doi: 10.1084/jem.159.6.1686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Petersen E., Høgh B., Marbiah N. T., Perlmann H., Willcox M., Dolopaie E., Hanson A. P., Björkman A., Perlmann P. A longitudinal study of antibodies to the Plasmodium falciparum antigen Pf155/RESA and immunity to malaria infection in adult Liberians. Trans R Soc Trop Med Hyg. 1990 May-Jun;84(3):339–345. doi: 10.1016/0035-9203(90)90307-z. [DOI] [PubMed] [Google Scholar]
  13. Quakyi I. A. The development and validation of an enzyme linked immunosorbent assay for malaria. Tropenmed Parasitol. 1980 Sep;31(3):325–333. [PubMed] [Google Scholar]
  14. Riley E. M., Allen S. J., Troye-Blomberg M., Bennett S., Perlmann H., Andersson G., Smedman L., Perlmann P., Greenwood B. M. Association between immune recognition of the malaria vaccine candidate antigen Pf155/RESA and resistance to clinical disease: a prospective study in a malaria-endemic region of west Africa. Trans R Soc Trop Med Hyg. 1991 Jul-Aug;85(4):436–443. doi: 10.1016/0035-9203(91)90207-f. [DOI] [PubMed] [Google Scholar]
  15. Thammapalerd N., Tharavanij S., Patarapotikul J., Looareesuwan S. Immunoprecipitating antibodies against blood stage antigens of Plasmodium falciparum in cerebral and non-cerebral malaria patients. Southeast Asian J Trop Med Public Health. 1987 Jun;18(2):171–178. [PubMed] [Google Scholar]
  16. Wahlgren M., Björkman A., Perlmann H., Berzins K., Perlmann P. Anti-Plasmodium falciparum antibodies acquired by residents in a holoendemic area of Liberia during development of clinical immunity. Am J Trop Med Hyg. 1986 Jan;35(1):22–29. doi: 10.4269/ajtmh.1986.35.22. [DOI] [PubMed] [Google Scholar]
  17. Wåhlin B., Wahlgren M., Perlmann H., Berzins K., Björkman A., Patarroyo M. E., Perlmann P. Human antibodies to a Mr 155,000 Plasmodium falciparum antigen efficiently inhibit merozoite invasion. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7912–7916. doi: 10.1073/pnas.81.24.7912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yount W. J., Seligmann M., Hong R., Good R., Kunkel H. G. Imbalances of gamma globulin subgroups and gene defects in patients with primary hypogammaglobulinemia. J Clin Invest. 1970 Nov;49(11):1957–1966. doi: 10.1172/JCI106415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES