Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1993 Oct;61(10):4510–4513. doi: 10.1128/iai.61.10.4510-4513.1993

Mitomycin-induced synthesis of a Shiga-like toxin from enteropathogenic Escherichia coli H.I.8.

A J Yee 1, S De Grandis 1, C L Gyles 1
PMCID: PMC281191  PMID: 8406845

Abstract

Escherichia coli H.I.8, an O128 infant diarrhea isolate, produces low titers of a unique Shiga-like toxin (SLT), called SLT-IIva, which is a variant of SLT-II. We investigated induction of toxin synthesis and the putative association of a bacteriophage with toxin synthesis. Induction of broth cultures of strain H.I.8 with mitomycin yielded a 3,000-fold increase in SLT-IIva, production of a colicin, and appearance of a bacteriophage. Southern hybridization demonstrated that the genes for SLT-IIva were not carried by the bacteriophage.

Full text

PDF
4510

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Farkas-Himsley H., Jessop J., Corey P. Development and standardization of a cytotoxic micro-assay for detection of enterotoxins: survey of enterotoxins from Escherichia coli of infant origin. Microbios. 1978;18(73-74):195–212. [PubMed] [Google Scholar]
  2. Gannon V. P., Gyles C. L. Characteristics of the Shiga-like toxin produced by Escherichia coli associated with porcine edema disease. Vet Microbiol. 1990 Jul;24(1):89–100. doi: 10.1016/0378-1135(90)90054-y. [DOI] [PubMed] [Google Scholar]
  3. Gannon V. P., Teerling C., Masri S. A., Gyles C. L. Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin II family. J Gen Microbiol. 1990 Jun;136(6):1125–1135. doi: 10.1099/00221287-136-6-1125. [DOI] [PubMed] [Google Scholar]
  4. Gemski P., O'Brien A. D., Wohlhieter J. A. Cellular release of heat-labile enterotoxin of Escherichia coli by bacteriophage induction. Infect Immun. 1978 Mar;19(3):1076–1082. doi: 10.1128/iai.19.3.1076-1082.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gyles C. L., De Grandis S. A., MacKenzie C., Brunton J. L. Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coli. Microb Pathog. 1988 Dec;5(6):419–426. doi: 10.1016/0882-4010(88)90003-4. [DOI] [PubMed] [Google Scholar]
  6. Hull A. E., Acheson D. W., Echeverria P., Donohue-Rolfe A., Keusch G. T. Mitomycin immunoblot colony assay for detection of Shiga-like toxin-producing Escherichia coli in fecal samples: comparison with DNA probes. J Clin Microbiol. 1993 May;31(5):1167–1172. doi: 10.1128/jcm.31.5.1167-1172.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson W. M., Pollard D. R., Lior H., Tyler S. D., Rozee K. R. Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction. J Clin Microbiol. 1990 Oct;28(10):2351–2353. doi: 10.1128/jcm.28.10.2351-2353.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Konowalchuk J., Dickie N., Stavric S., Speirs J. I. Comparative studies of five heat-labile toxic products of Escherichia coli. Infect Immun. 1978 Dec;22(3):644–648. doi: 10.1128/iai.22.3.644-648.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Konowalchuk J., Speirs J. I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun. 1977 Dec;18(3):775–779. doi: 10.1128/iai.18.3.775-779.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lown J. W., Begleiter A., Johnson D., Morgan A. R. Studies related to antitumor antibiotics. Part V. Reactions of mitomycin C with DNA examined by ethidium fluorescence assay. Can J Biochem. 1976 Feb;54(2):110–119. doi: 10.1139/o76-018. [DOI] [PubMed] [Google Scholar]
  11. MacLeod D. L., Gyles C. L. Purification and characterization of an Escherichia coli Shiga-like toxin II variant. Infect Immun. 1990 May;58(5):1232–1239. doi: 10.1128/iai.58.5.1232-1239.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Molina N. C., Peterson J. W. Cholera toxin-like toxin released by Salmonella species in the presence of mitomycin C. Infect Immun. 1980 Oct;30(1):224–230. doi: 10.1128/iai.30.1.224-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Brien A. D., LaVeck G. D., Thompson M. R., Formal S. B. Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. J Infect Dis. 1982 Dec;146(6):763–769. doi: 10.1093/infdis/146.6.763. [DOI] [PubMed] [Google Scholar]
  14. O'Brien A. D., Newland J. W., Miller S. F., Holmes R. K., Smith H. W., Formal S. B. Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science. 1984 Nov 9;226(4675):694–696. doi: 10.1126/science.6387911. [DOI] [PubMed] [Google Scholar]
  15. Peterson J. W., Houston C. W., Koo F. C. Influence of cultural conditions on mitomycin C-mediated bacteriophage induction and release of Salmonella toxin. Infect Immun. 1981 Apr;32(1):232–242. doi: 10.1128/iai.32.1.232-242.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pollard D. R., Johnson W. M., Lior H., Tyler S. D., Rozee K. R. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J Clin Microbiol. 1990 Mar;28(3):540–545. doi: 10.1128/jcm.28.3.540-545.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmitt C. K., McKee M. L., O'Brien A. D. Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511. Infect Immun. 1991 Mar;59(3):1065–1073. doi: 10.1128/iai.59.3.1065-1073.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scotland S. M., Smith H. R., Willshaw G. A., Rowe B. Vero cytotoxin production in strain of Escherichia coli is determined by genes carried on bacteriophage. Lancet. 1983 Jul 23;2(8343):216–216. doi: 10.1016/s0140-6736(83)90192-7. [DOI] [PubMed] [Google Scholar]
  19. Smith H. W., Green P., Parsell Z. Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. J Gen Microbiol. 1983 Oct;129(10):3121–3137. doi: 10.1099/00221287-129-10-3121. [DOI] [PubMed] [Google Scholar]
  20. Strockbine N. A., Marques L. R., Newland J. W., Smith H. W., Holmes R. K., O'Brien A. D. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun. 1986 Jul;53(1):135–140. doi: 10.1128/iai.53.1.135-140.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Takeda Y., Murphy J. R. Bacteriophage conversion of heat-labile enterotoxin in Escherichia coli. J Bacteriol. 1978 Jan;133(1):172–177. doi: 10.1128/jb.133.1.172-177.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Takeda Y., Okamoto K., Miwatani T. Mitomycin C stimulates production of a toxin in Shigella species that causes morphological changes in Chinese hamster ovary cells. Infect Immun. 1979 Jan;23(1):178–180. doi: 10.1128/iai.23.1.178-180.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES