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. 1993 Dec;61(12):5326–5332. doi: 10.1128/iai.61.12.5326-5332.1993

Presentation of mycobacterial antigens by human dendritic cells: lack of transfer from infected macrophages.

P Pancholi 1, A Mirza 1, V Schauf 1, R M Steinman 1, N Bhardwaj 1
PMCID: PMC281318  PMID: 7693599

Abstract

When exposed to a challenge of 10 Mycobacterium bovis BCG cells per antigen-presenting cell, most human monocytes engulf several organisms. In contrast, blood dendritic cells which are potent antigen-presenting cells for several antigens are not detectably phagocytic for mycobacteria. We investigated the possibility that infected macrophages might regurgitate antigens for presentation by populations of human blood dendritic cells. Macrophages were infected with M. bovis BCG, mixed with uninfected dendritic cells, and added to immune T cells, either bulk T cells or cloned populations from BCG vaccinees or patients recovering from tuberculosis. The macrophages were from donors who were mismatched to the T cells so that transfer of antigen to major histocompatibility complex-matched dendritic cells could be evaluated. As we describe, there was no evidence for the transfer of mycobacterial antigens from macrophages to dendritic cells in a form that was stimulatory for the T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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