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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Oncogene. 2009 Nov 2;29(4):589–596. doi: 10.1038/onc.2009.366

Figure 3.

Figure 3

Notch3-mediated apoptosis is dependent on Bim in vitro. (A) Twenty-four hours after Notch3 siRNA transfection, lung cancer cell line HCC2429 was transfected with Bim siRNA. Cells were harvested after an additional 24 hours. Reduction of Bim expression was observed after 24 hours as compared control siRNA. The loss of Bim prevented induction of PARP by Notch3 siRNA. (B) In serum free media, the loss of Bim abrogates apoptosis, suggesting that Bim is necessary for Notch3-dependent apoptosis. No significant change is observed in 10% FCS condition. In all siRNA experiments, non-silencing siRNA was used as control. The difference between SiRNA-C and Bim SiRNA in N3SiRNA treated cells were statistical significance with p < 0.05.