Fig. 1.

Adult oligodendrocyte precursor cell (adult OPC) cultures are not contaminated with SCs. (A) Phase contrast microscopy shows that adult OPCs exhibit several processes emanating from a small round cell body (inset shows higher magnification view of boxed cell). (B) Nearly all cells express A2B5, as demonstrated by immunohistochemistry. FACS analysis shows uniform expression of the glial precursor markers A2B5 (98%) and NG2 (93%) and an absence of p75 expression by adult OPC cultures (C–E). In contrast to adult OPCs, SCs do not express A2B5 or NG2 but greater than 95% express p75 (F–H). (I) Immunohistochemistry confirms a lack of p75 expression by adult OPCs while SCs demonstrate uniform expression (J). (C–H) The green plot represents unlabeled control cells while the purple plot represents the population of cells labeled with fluorescent antibody. (C–H) Data are representative of three independent experiments. Scale bar = 24 μm (A); 18 μm(B); 28 μm (I, J).