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. 1993 Apr;61(4):1272–1280. doi: 10.1128/iai.61.4.1272-1280.1993

Immunochemistry of capsular type polysaccharide and virulence properties of type VI Streptococcus agalactiae (group B streptococci).

C von Hunolstein 1, S D'Ascenzi 1, B Wagner 1, J Jelínková 1, G Alfarone 1, S Recchia 1, M Wagner 1, G Orefici 1
PMCID: PMC281358  PMID: 8454331

Abstract

The immunochemistry of capsular type polysaccharide and virulence characteristics of group B streptococci (GBS), type VI, were studied. By high-pressure anion-exchange chromatography and pulsed amperometric detection, as well as by 13C nuclear magnetic resonance analysis, both extracellular and cell-bound polysaccharides were found to contain glucose, galactose, and N-acetylneuraminic acid in the molar ratio of 2:2:1, respectively. At variance with all other GBS serotypes described to date (Ia, Ib, II, III, IV, and V), no N-acetylglucosamine was present, whatever the source of the material (secreted or cell bound; reference or clinical isolate). Sialic acid was probably involved in the immunodeterminant structure of this new serotype since cleavage of this sugar from the polysaccharide gave rise to an antigen which reacted very weakly with type VI antiserum and to a precipitation line in immunodiffusion with no identity with the native type VI polysaccharide. By using type VI antiserum and the protein A-gold technique, a large capsule was observed in the type VI GBS reference strain by electron microscopy. All type VI strains examined were lethal for CD-1 mice, the 50% lethal dose after intraperitoneal challenge ranging from 1.0 (+/- 0.9, standard deviation) x 10(5) to 2.5 (+/- 1.5, standard deviation) x 10(5) CFU per mouse. A rabbit antiserum against capsular type polysaccharide exhibited both protective activity for mice injected intraperitoneally with type VI reference strain or with clinical isolates and opsonic activity in a phagocytosis assay.

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Selected References

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  1. Baker C. J., Edwards M. S. Group B streptococcal infections. Perinatal impact and prevention methods. Ann N Y Acad Sci. 1988;549:193–202. doi: 10.1111/j.1749-6632.1988.tb23972.x. [DOI] [PubMed] [Google Scholar]
  2. Baltimore R. S., Kasper D. L., Baker C. J., Goroff D. K. Antigenic specificity of opsonophagocytic antibodies in rabbit anti-sera to group B streptococci. J Immunol. 1977 Feb;118(2):673–678. [PubMed] [Google Scholar]
  3. Baltimore R. S., Kasper D. L., Vecchitto J. Mouse protection test for group B Streptococcus type III. J Infect Dis. 1979 Jul;140(1):81–88. doi: 10.1093/infdis/140.1.81. [DOI] [PubMed] [Google Scholar]
  4. Berman E. Structural and conformational analysis of sialyloligosaccharides using carbon-13 nuclear magnetic resonance spectroscopy. Biochemistry. 1984 Jul 31;23(16):3754–3759. doi: 10.1021/bi00311a029. [DOI] [PubMed] [Google Scholar]
  5. Bhattacharjee A. K., Jennings H. J., Kenny C. P., Martin A., Smith I. C. Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem. 1975 Mar 10;250(5):1926–1932. [PubMed] [Google Scholar]
  6. Brossmer R., Wagner M., Fischer E. Specificity of the sialic acid-binding lectin from the snail Cepaea hortensis. J Biol Chem. 1992 May 5;267(13):8752–8756. [PubMed] [Google Scholar]
  7. Cleat P. H., Coid C. R. Phagocytic and bactericidal activity of human neutrophils against two isolates of Group B streptococci Type Ic of differing pathogenicity. Br J Exp Pathol. 1981 Aug;62(4):393–397. [PMC free article] [PubMed] [Google Scholar]
  8. Edwards M. S., Nicholson-Weller A., Baker C. J., Kasper D. L. The role of specific antibody in alternative complement pathway-mediated opsonophagocytosis of type III, group B Streptococcus. J Exp Med. 1980 May 1;151(5):1275–1287. doi: 10.1084/jem.151.5.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferrieri P. Neonatal susceptibility and immunity to major bacterial pathogens. Rev Infect Dis. 1990 May-Jun;12 (Suppl 4):S394–S400. doi: 10.1093/clinids/12.supplement_4.s394. [DOI] [PubMed] [Google Scholar]
  10. Hardy M. R., Townsend R. R., Lee Y. C. Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection. Anal Biochem. 1988 Apr;170(1):54–62. doi: 10.1016/0003-2697(88)90089-9. [DOI] [PubMed] [Google Scholar]
  11. Holm S. E., Bergholm A. M., Wagner B., Wagner M. A sialic-acid-specific lectin from Cepaea hortensis that promotes phagocytosis of a group-B, type-Ia, streptococcal strain. J Med Microbiol. 1985 Jun;19(3):317–323. doi: 10.1099/00222615-19-3-317. [DOI] [PubMed] [Google Scholar]
  12. Jelínková J., Motlová J. Worldwide distribution of two new serotypes of group B streptococci: type IV and provisional type V. J Clin Microbiol. 1985 Mar;21(3):361–362. doi: 10.1128/jcm.21.3.361-362.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jennings H. J., Katzenellenbogen E., Lugowski C., Kasper D. L. Structure of native polysaccharide antigens of type Ia and type Ib group B Streptococcus. Biochemistry. 1983 Mar 1;22(5):1258–1264. doi: 10.1021/bi00274a042. [DOI] [PubMed] [Google Scholar]
  14. Jennings H. J., Rosell K. G., Kasper D. L. Structural determination and serology of the native polysaccharide antigen of type-III group B Streptococcus. Can J Biochem. 1980 Feb;58(2):112–120. doi: 10.1139/o80-016. [DOI] [PubMed] [Google Scholar]
  15. Jennings H. J., Rosell K. G., Katzenellenbogen E., Kasper D. L. Structural determination of the capsular polysaccharide antigen of type II group B Streptococcus. J Biol Chem. 1983 Feb 10;258(3):1793–1798. [PubMed] [Google Scholar]
  16. Kasper D. L. Bacterial capsule--old dogmas and new tricks. J Infect Dis. 1986 Mar;153(3):407–415. doi: 10.1093/infdis/153.3.407. [DOI] [PubMed] [Google Scholar]
  17. Lancefield R. C. A SEROLOGICAL DIFFERENTIATION OF SPECIFIC TYPES OF BOVINE HEMOLYTIC STREPTOCOCCI (GROUP B). J Exp Med. 1934 Mar 31;59(4):441–458. doi: 10.1084/jem.59.4.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Levy N. J., Kasper D. L. Antibody-independent and -dependent opsonization of group B Streptococcus requires the first component of complement C1. Infect Immun. 1985 Jul;49(1):19–24. doi: 10.1128/iai.49.1.19-24.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mackie E. B., Brown K. N., Lam J., Costerton J. W. Morphological stabilization of capsules of group B streptococci, types Ia, Ib, II, and III, with specific antibody. J Bacteriol. 1979 May;138(2):609–617. doi: 10.1128/jb.138.2.609-617.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  21. Opal S. M., Cross A., Palmer M., Almazan R. Group B streptococcal sepsis in adults and infants. Contrasts and comparisons. Arch Intern Med. 1988 Mar;148(3):641–645. [PubMed] [Google Scholar]
  22. Orefici G., Recchia S., Galante L. Possible virulence marker for Streptococcus agalactiae (Lancefield Group B). Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):302–305. doi: 10.1007/BF01963108. [DOI] [PubMed] [Google Scholar]
  23. Perch B., Kjems E., Henrichsen New serotypes of group B streptococci isolated from human sources. J Clin Microbiol. 1979 Jul;10(1):109–110. doi: 10.1128/jcm.10.1.109-110.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pluschke G., Mayden J., Achtman M., Levine R. P. Role of the capsule and the O antigen in resistance of O18:K1 Escherichia coli to complement-mediated killing. Infect Immun. 1983 Dec;42(3):907–913. doi: 10.1128/iai.42.3.907-913.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Quinti I., Papetti C., von Hunolstein C., Orefici G., Aiuti F. IgG subclasses to group B streptococci in normals, colonized woman and IgG2 subclass-deficient patients. Monogr Allergy. 1988;23:148–155. [PubMed] [Google Scholar]
  26. Rýc M., Jelínková J., Motlová J., Wagner M. Immuno-electronmicroscopic demonstration of capsules on group-B streptococci of new serotypes and type candidates. J Med Microbiol. 1988 Feb;25(2):147–149. doi: 10.1099/00222615-25-2-147. [DOI] [PubMed] [Google Scholar]
  27. Shigeoka A. O., Hall R. T., Hill H. R. Strain specificity of opsonins for group B streptococci types II and III. Infect Immun. 1979 Feb;23(2):438–445. doi: 10.1128/iai.23.2.438-445.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Shigeoka A. O., Rote N. S., Santos J. I., Hill H. R. Assessment of the virulence factors of group B streptococci: correlation with sialic acid content. J Infect Dis. 1983 May;147(5):857–863. doi: 10.1093/infdis/147.5.857. [DOI] [PubMed] [Google Scholar]
  29. Stanton B. F., Baltimore R. S., Shedd D. G. Effects of route and time of administration of antiserum on protection of mice from lethal infection due to group B Streptococcus type III. Infect Immun. 1981 Jan;31(1):391–395. doi: 10.1128/iai.31.1.391-395.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tissi L., Marconi P., Mosci P., Merletti L., Cornacchione P., Rosati E., Recchia S., von Hunolstein C., Orefici G. Experimental model of type IV Streptococcus agalactiae (group B streptococcus) infection in mice with early development of septic arthritis. Infect Immun. 1990 Sep;58(9):3093–3100. doi: 10.1128/iai.58.9.3093-3100.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  32. Wagner B., Atrat P. G., Clark-Curtiss J. E., Wagner M. Localization of the steroid 1-dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy. J Basic Microbiol. 1992;32(1):65–71. doi: 10.1002/jobm.3620320119. [DOI] [PubMed] [Google Scholar]
  33. Wessels M. R., Benedí W. J., Jennings H. J., Michon F., DiFabio J. L., Kasper D. L. Isolation and characterization of type IV group B Streptococcus capsular polysaccharide. Infect Immun. 1989 Apr;57(4):1089–1094. doi: 10.1128/iai.57.4.1089-1094.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wessels M. R., DiFabio J. L., Benedì V. J., Kasper D. L., Michon F., Brisson J. R., Jelínková J., Jennings H. J. Structural determination and immunochemical characterization of the type V group B Streptococcus capsular polysaccharide. J Biol Chem. 1991 Apr 15;266(11):6714–6719. [PubMed] [Google Scholar]
  35. Wessels M. R., Rubens C. E., Benedí V. J., Kasper D. L. Definition of a bacterial virulence factor: sialylation of the group B streptococcal capsule. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8983–8987. doi: 10.1073/pnas.86.22.8983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wibawan I. W., Lämmler C. Influence of capsular neuraminic acid on properties of streptococci of serological group B. J Gen Microbiol. 1991 Dec;137(12):2721–2725. doi: 10.1099/00221287-137-12-2721. [DOI] [PubMed] [Google Scholar]
  37. Wilkinson H. W. Detection of group B streptococcal antibodies in human sera by radioimmunoassay: concentrations of type-specific antibodies in sera of adults and infants infected with group B streptococci. J Clin Microbiol. 1978 Feb;7(2):194–201. doi: 10.1128/jcm.7.2.194-201.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Wilkinson H. W. Nontypable group B streptococci isolated from human sources. J Clin Microbiol. 1977 Aug;6(2):183–184. doi: 10.1128/jcm.6.2.183-184.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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