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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Nov 30;397(1):231. doi: 10.1016/j.virol.2009.11.014

Figure 4. TLR3 signaling increases RIG-I expression, but is not dependent on IFN signaling.

Figure 4

A and B) dsRNA increases RIG-I expression. 293 cells expressing TLR3 or LacZ (control) were treated without (−) or with (+) exogenous dsRNA (PolyI:C; 5 ug/ml). After 18 h, lysates were harvested and analyzed by Western blotting for levels of RIG-I and actin (panel A) or by RT-PCR for levels of RIG-I and GAPDH RNA. C) IFN-beta secretion. 293 cells expressing TLR3 or LacZ were treated without (−) or with (+) 5 ug/ml PolyI:C dsRNA and after 18 h media was collected and analyzed by ELISA for IFN-beta. Media from A549 cells infected with P/V-CPI- was used as a positive control. Data are from triplicate samples and error bars represent standard deviation. D) STAT1 levels. 293-TLR3 cells were mock infected or infected at an moi of 10 with the indicated viruses. At 6 h pi, cells were left untreated or treated with (+) dsRNA. Six hr later, cell lysates were analyzed by western blotting for STAT1 and actin.