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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Toxicon. 2009 Oct 21;55(2-3):619. doi: 10.1016/j.toxicon.2009.10.019

Fig. 3.

Fig. 3

Lipid-based DNA transfection reagents promote cellular internalization of BoNT/A Lc protease in the absence of Hc. Neuronal cell lines N2A, M17 and non-neuronal cell lines HIT-T15, HEK293 (293) were exposed for 24 hrs to 30 nM of recombinant BoNT/A LC protease, using either the full-length protease (Lc438) or the carboxyl-truncated form (Lc424). The protease was added to cell culture with (+) or without (−) pre-incubation with the DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.