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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Toxicon. 2009 Oct 21;55(2-3):619. doi: 10.1016/j.toxicon.2009.10.019

Fig. 6.

Fig. 6

DNA transfection reagent enhances internalization of multiple BoNT Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with FuGene-HD. Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.