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. 2010 Feb;12(2):128–138. doi: 10.1593/neo.91438

Figure 4.

Figure 4

Signaling pathways for HGF-induced anoikis resistance and chemoresistance. (A) CaOV-3 and SKOV-3 cells plated on culture dishes (adherent) or polyHEMA-coated dishes (nonadherent) were pretreated with 200 nM wortmannin (Wt) or 50 µM PD98059 (PD) for 30 minutes and (B) subjected to 50 µM cisplatin (CDDP) or 100 nM paclitaxel (PTX) treatment in the presence or absence of 10 ng/ml HGF for 72 hours. Cell apoptosis was determined with the TUNEL assay. The number of apoptotic cells was counted, and the bar diagram summarized the percentage of apoptotic cells from triplicate determination. Error bars indicate the SD of the mean. *P < .05 versus untreated controls.