Table 2.
Frequencies1 of HA specific MBC before and after vaccination with HA/A/VN/1203/04
| Day | H5/HK/156/97 | H5/VN/1203/04 | H1/NC/99 | H3/WY/99 | H6/Teal/97 | control |
|---|---|---|---|---|---|---|
| 0 | 0.8 ± 1.5 | 0.8 ± 1.3 | 0.6 ± 1.1 | 1.4 ± 1.9 | 0.5 ± 1.1 | 0.1 ± 0.1 |
| 7 | 2.5 ± 3.42 | 2.3 ± 2.82 | 1.3 ± 1.8 | 1.3 ± 1.8 | 0.4 ± 0.5 | 0.0 ± 0.1 |
| 28 | 1.0 ± 1.5 | 1.3 ± 2.3 | 0.5 ± 0.6 | 0.7 ± 0.8 | 0.2 ± 0.4 | 0.0 ± 0.0 |
| 56 | 1.9 ± 2.2 | 1.7 ± 2.1 | 2.2 ± 3.6 | 1.7 ± 2.7 | 0.6 ± 1.3 | 0.1 ± 0.2 |
1
MBC IgG ELISPOT assays were conducted as described in Materials and Methods in plates coated with the indicated rHA proteins or a capture antibody for human IgG. The frequency of HA specific B cells is calculated by dividing the number of spots against each HA, by the number of total IgG spots at the same cell concentration. The resulting values were multiplied by 100 to give a percent HA/total IgG (100 × HA/total IgG = % HA specific). Averages and standard deviations were determined for the entire cohort of 28 subjects.
2
p < 0.01