TABLE 2.
Mutant terminases with broad packaging specificity
| Line | Helper | Passive prophage | Prophage yielda (SEM)b | Passive prophage | Prophage yielda (SEM)b | Discrimination indexc |
|---|---|---|---|---|---|---|
| 1 | λ-P1 Nu1+ | λ | 1.02 (0.36) | 21 | 3.24 (1.37) × 10−5 | 3.1 × 104 |
| 2 | λ-P1 Nu1hy51 (H21 T21H21 w21) | λ | 8.02 (0.9) × 10−5 | 21 | 2.62 (0.29) | 3.3 × 104 |
| 3 | λ-P1 Nu1hy2 (H21 T21H21 wλ) | λ | 2.9 (0.55) × 10−6 | 21 | 2.15 (0.175) | 7.4 × 105 |
| 4 | λ-P1 Nu1hy1 (Hλ TλH21 wλ) | λ | 0.27 (0.040) | 21 | 2.86 (0.40) | 1.1 × 101 |
| 5 | λ-P1 Nu1hy1E20D | λ | 0.36 (0.14) | 21 | 0.41 (0.17) | 1.1 |
| 6 | λ-P1 Nu1hy1E20G | λ | 1.03 (0.14) | 21 | 1.90 (0.35) | 1.8 |
| 7 | λ-P1 Nu1hy1Q23R | λ | 0.70 (0.14) | 21 | 1.12 (0.22) | 1.6 |
| 8 | λ-P1 Nu1hy1Q23K | λ | 1.35 (0.18) | 21 | 1.29 (0.21) | 0.95 |
| 9 | λ-P1 Nu1hy1L40I | λ | 0.90 (0.20) | 21 | 2.55 (0.17) | 2.8 |
| 10 | λ-P1 Nu1hy3 (H21 TλH21 wλ) | λ | 0.03 (0.006) | 21 | 1.63 (0.24) | 54 |
| 11 |
λ-P1 Nu1hy4 (Hλ T21H21 wλ) |
λ |
0.15 (0.04) |
21 |
1.72 (0.33) |
11 |
a
Plaque-forming units/induced lysogen.
b
Values in parentheses are the standard error of the mean (3 ≤ n ≤ 5).
c
Discrimination index is the yield of the cognate passive prophage/yield of the noncognate passive prophage.