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. 1988 Aug;85(15):5497–5501. doi: 10.1073/pnas.85.15.5497

Antibody to sigma 32 cross-reacts with DnaK: association of DnaK protein with Escherichia coli RNA polymerase.

S Skelly 1, C F Fu 1, B Dalie 1, B Redfield 1, T Coleman 1, N Brot 1, H Weissbach 1
PMCID: PMC281784  PMID: 3041413

Abstract

A polyclonal antibody to sigma 32, the heat shock sigma factor, has been used to show the presence of low levels of sigma 32 in Escherichia coli RNA polymerase preparations (E sigma 70), which explains the observed in vitro activity of E sigma 70 towards heat shock genes. The sigma 32 antibody cross-reacts with DnaK, and DnaK has been found associated with purified preparations of both E sigma 70 and the heat shock RNA polymerase, E sigma 32.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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