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. 1988 Aug;85(15):5738–5742. doi: 10.1073/pnas.85.15.5738

Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

J R Duguid 1, R G Rohwer 1, B Seed 1
PMCID: PMC281836  PMID: 2456582

Abstract

We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.

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