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. 1988 Aug;85(16):5834–5838. doi: 10.1073/pnas.85.16.5834

A repressor heterodimer binds to a chimeric operator.

M Hollis 1, D Valenzuela 1, D Pioli 1, R Wharton 1, M Ptashne 1
PMCID: PMC281859  PMID: 3413061

Abstract

Replacement of the solvent-exposed residues of the DNA recognition helix of the 434 repressor with the corresponding residues of the P22 repressor generates a hybrid protein, 434R[alpha 3(P22R)], which binds specifically to P22 operators. We show here that a new DNA-binding specificity is generated by combining 434 and 434R[alpha 3(P22R)] repressor monomers to form a heterodimer. The heterodimer specifically recognizes a chimeric P22/434 operator that lacks two-fold rotational symmetry.

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Selected References

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