Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Aug;85(16):5889–5893. doi: 10.1073/pnas.85.16.5889

Parathyroid hormone modulates transforming growth factor beta activity and binding in osteoblast-enriched cell cultures from fetal rat parietal bone.

M Centrella 1, T L McCarthy 1, E Canalis 1
PMCID: PMC281870  PMID: 2901093

Abstract

Transforming growth factor beta (TGF-beta) is produced by bone cells, is abundant in bone matrix, and regulates bone cell biochemical processes. In osteoblast-enriched fetal rat parietal bone cell cultures, low TGF-beta doses increase DNA synthesis, whereas higher levels are less mitogenic, stimulate collagen production, and decrease alkaline phosphatase activity. Parathyroid hormone by itself has minimal effects on these processes, but it opposes the effects of TGF-beta and alters TGF-beta binding to its receptors in osteoblast-enriched cultures. Some functions ascribed to parathyroid hormone in bone may therefore result from alterations in TGF-beta activity, suggesting that the local effects of TGF-beta in bone are under systemic hormonal control.

Full text

PDF
5889

Images in this article

5891Image
on p.5891
5892Image
on p.5892

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Centrella M., Canalis E. Isolation of EGF-dependent transforming growth factor (TGF beta-like) activity from culture medium conditioned by fetal rat calvariae. J Bone Miner Res. 1987 Feb;2(1):29–36. doi: 10.1002/jbmr.5650020106. [DOI] [PubMed] [Google Scholar]
  2. Centrella M., Canalis E. Local regulators of skeletal growth: a perspective. Endocr Rev. 1985 Fall;6(4):544–551. doi: 10.1210/edrv-6-4-544. [DOI] [PubMed] [Google Scholar]
  3. Centrella M., Canalis E. Transforming and nontransforming growth factors are present in medium conditioned by fetal rat calvariae. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7335–7339. doi: 10.1073/pnas.82.21.7335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Centrella M., McCarthy T. L., Canalis E. Mitogenesis in fetal rat bone cells simultaneously exposed to type beta transforming growth factor and other growth regulators. FASEB J. 1987 Oct;1(4):312–317. doi: 10.1096/fasebj.1.4.3498658. [DOI] [PubMed] [Google Scholar]
  5. Centrella M., McCarthy T. L., Canalis E. Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. J Biol Chem. 1987 Feb 25;262(6):2869–2874. [PubMed] [Google Scholar]
  6. Cheifetz S., Like B., Massagué J. Cellular distribution of type I and type II receptors for transforming growth factor-beta. J Biol Chem. 1986 Jul 25;261(21):9972–9978. [PubMed] [Google Scholar]
  7. Fanger B. O., Wakefield L. M., Sporn M. B. Structure and properties of the cellular receptor for transforming growth factor type beta. Biochemistry. 1986 Jun 3;25(11):3083–3091. doi: 10.1021/bi00359a003. [DOI] [PubMed] [Google Scholar]
  8. Gunness-Hey M., Hock J. M. Increased trabecular bone mass in rats treated with human synthetic parathyroid hormone. Metab Bone Dis Relat Res. 1984;5(4):177–181. doi: 10.1016/0221-8747(84)90026-2. [DOI] [PubMed] [Google Scholar]
  9. Hauschka P. V., Mavrakos A. E., Iafrati M. D., Doleman S. E., Klagsbrun M. Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose. J Biol Chem. 1986 Sep 25;261(27):12665–12674. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lieberherr M. Effects of vitamin D3 metabolites on cytosolic free calcium in confluent mouse osteoblasts. J Biol Chem. 1987 Sep 25;262(27):13168–13173. doi: 10.1515/9783110846713.769. [DOI] [PubMed] [Google Scholar]
  12. Luben R. A., Wong G. L., Cohn D. V. Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts. Endocrinology. 1976 Aug;99(2):526–534. doi: 10.1210/endo-99-2-526. [DOI] [PubMed] [Google Scholar]
  13. Massagué J., Like B. Cellular receptors for type beta transforming growth factor. Ligand binding and affinity labeling in human and rodent cell lines. J Biol Chem. 1985 Mar 10;260(5):2636–2645. [PubMed] [Google Scholar]
  14. Massagué J. Type beta transforming growth factor from feline sarcoma virus-transformed rat cells. Isolation and biological properties. J Biol Chem. 1984 Aug 10;259(15):9756–9761. [PubMed] [Google Scholar]
  15. McSheehy P. M., Chambers T. J. Osteoblastic cells mediate osteoclastic responsiveness to parathyroid hormone. Endocrinology. 1986 Feb;118(2):824–828. doi: 10.1210/endo-118-2-824. [DOI] [PubMed] [Google Scholar]
  16. Peterkofsky B., Diegelmann R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971 Mar 16;10(6):988–994. doi: 10.1021/bi00782a009. [DOI] [PubMed] [Google Scholar]
  17. Pfeilschifter J., Mundy G. R. Modulation of type beta transforming growth factor activity in bone cultures by osteotropic hormones. Proc Natl Acad Sci U S A. 1987 Apr;84(7):2024–2028. doi: 10.1073/pnas.84.7.2024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pliam N. B., Nyiredy K. O., Arnaud C. D. Parathyroid hormone receptors in avian bone cells. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2061–2063. doi: 10.1073/pnas.79.6.2061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Podbesek R., Edouard C., Meunier P. J., Parsons J. A., Reeve J., Stevenson R. W., Zanelli J. M. Effects of two treatment regimes with synthetic human parathyroid hormone fragment on bone formation and the tissue balance of trabecular bone in greyhounds. Endocrinology. 1983 Mar;112(3):1000–1006. doi: 10.1210/endo-112-3-1000. [DOI] [PubMed] [Google Scholar]
  20. Robey P. G., Young M. F., Flanders K. C., Roche N. S., Kondaiah P., Reddi A. H., Termine J. D., Sporn M. B., Roberts A. B. Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro. J Cell Biol. 1987 Jul;105(1):457–463. doi: 10.1083/jcb.105.1.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rodan G. A., Martin T. J. Role of osteoblasts in hormonal control of bone resorption--a hypothesis. Calcif Tissue Int. 1981;33(4):349–351. doi: 10.1007/BF02409454. [DOI] [PubMed] [Google Scholar]
  22. Seyedin S. M., Thompson A. Y., Bentz H., Rosen D. M., McPherson J. M., Conti A., Siegel N. R., Galluppi G. R., Piez K. A. Cartilage-inducing factor-A. Apparent identity to transforming growth factor-beta. J Biol Chem. 1986 May 5;261(13):5693–5695. [PubMed] [Google Scholar]
  23. Wong G. L. Paracrine interactions in bone-secreted products of osteoblasts permit osteoclasts to respond to parathyroid hormone. J Biol Chem. 1984 Apr 10;259(7):4019–4022. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES