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. 1988 Aug;85(16):5927–5931. doi: 10.1073/pnas.85.16.5927

Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry.

A C Brinegar 1, G Cooper 1, A Stevens 1, C R Hauer 1, J Shabanowitz 1, D F Hunt 1, J E Fox 1
PMCID: PMC281878  PMID: 3413067

Abstract

A wheat embryo cytokinin-binding protein was covalently modified with the radiolabeled photoaffinity ligand 2-azido-N6-[14C]benzyladenine. A single labeled peptide was obtained after proteolytic digestion and isolation by reversed-phase and anion-exchange HPLC. Sequencing by classical Edman degradation identified 11 of the 12 residues but failed to identify the labeled amino acid. Analysis by laser photodissociation Fourier-transform mass spectrometry of 10 pmol of the peptide independently confirmed the Edman data and also demonstrated that the histidine residue nearest the C terminus (underlined) was modified by the reagent in the sequence Ala-Phe-Leu-Gln-Pro-Ser-His-His-Asp-Ala-Asp-Glu.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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