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. 1988 Aug;85(16):6022–6026. doi: 10.1073/pnas.85.16.6022

Recognition and cleavage site of the intron-encoded omega transposase.

L Colleaux 1, L D'Auriol 1, F Galibert 1, B Dujon 1
PMCID: PMC281897  PMID: 2842757

Abstract

The optional group I intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae contains a 235-codon-long open reading frame the translation product of which (the omega transposase) catalyzes the formation of a double-strand break within the intron-minus (omega-) copies of the same gene. Purified omega transposase generates in vitro a 4-base-pair staggered cut with 3' hydroxyl overhangs at the exact position where the intron eventually inserts in the gene. Using randomly mutagenized synthetic oligonucleotides, single-base mutants were produced at 21 positions around the cleavage site. Experiments with these oligonucleotides show that the recognition site extends over an 18-base pair-long sequence within which minimal sequence degeneracy is tolerated. The intron-encoded omega transposase is, therefore, one of the most specific restriction endonucleases known to date.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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