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. 1988 Sep;85(17):6257–6261. doi: 10.1073/pnas.85.17.6257

Stimulation of protein phosphatase activity by insulin and growth factors in 3T3 cells.

C P Chan 1, S J McNall 1, E G Krebs 1, E H Fischer 1
PMCID: PMC281948  PMID: 2842761

Abstract

Incubation of Swiss mouse 3T3-D1 cells with physiological concentrations of insulin resulted in a rapid and transient activation of protein phosphatase activity as measured by using [32P]phosphorylase a as substrate. Activation reached a maximum level (140% of control value) within 5 min of addition and returned to control levels within 20 min. The effect of insulin was dose-dependent with half-maximal activation occurring at approximately 5 nM insulin. This activity could be completely inhibited by addition of the heat-stable protein inhibitor 2, which suggests the presence of an activated type-1 phosphatase. Similar effects on phosphatase activity were seen when epidermal growth factor and platelet-derived growth factor were tested. These results suggest that some of the intracellular effects caused by insulin and growth factors are mediated through the activation of a protein phosphatase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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