Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Sep;85(17):6366–6369. doi: 10.1073/pnas.85.17.6366

Interaction of a gibberellin-induced factor with the upstream region of an alpha-amylase gene in rice aleurone tissue.

T M Ou-Lee 1, R Turgeon 1, R Wu 1
PMCID: PMC281972  PMID: 3261867

Abstract

The interaction between the DNA sequences of an alpha-amylase (EC 3.2.1.1) gene and a tissue-specific factor induced in rice (Oryza sativa L.) aleurone tissue by gibberellin was studied. DNA mobility-shift during electrophoresis indicated that a 500-base-pair sequence (HS500) of a rice alpha-amylase genomic clone (OSamy-a) specifically interacted with a factor from gibberellin-induced rice aleurone tissue. The amount of complex formed between the HS500 DNA fragment and the gibberellin-induced factor increased in proportion to increasing amounts of aleurone extract and decreased with the addition of unlabeled HS500 DNA fragment. A specific segment of the HS500 fragment was protected from exonuclease III digestion by protein(s) in the aleurone extract, revealing the approximate position of the protein-DNA interaction. In the protected region, there is a direct repeat that overlaps with a potential stem-loop structure. Protein in extracts from leaves, roots, or deembryonated seeds incubated without gibberellin A3 did not bind to the HS500 DNA fragment. However, the binding factor was induced in deembryonated seeds when exogenous gibberellin A3 was added during seed imbibition. This suggests that activation of alpha-amylase synthesis by gibberellin may be mediated by the formation of a factor that interacts with a specific sequence of the alpha-amylase gene.

Full text

PDF
6366

Images in this article

6367Image
on p.6367
6367Image
on p.6367
6368Image
on p.6368

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baulcombe D., Lazarus C., Martienssen R. Gibberellins and gene control in cereal aleurone cells. J Embryol Exp Morphol. 1984 Nov;83 (Suppl):119–135. [PubMed] [Google Scholar]
  2. Carey J. Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study. Proc Natl Acad Sci U S A. 1988 Feb;85(4):975–979. doi: 10.1073/pnas.85.4.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen Z. L., Schuler M. A., Beachy R. N. Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8560–8564. doi: 10.1073/pnas.83.22.8560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colot V., Robert L. S., Kavanagh T. A., Bevan M. W., Thompson R. D. Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco. EMBO J. 1987;6(12):3559–3564. doi: 10.1002/j.1460-2075.1987.tb02685.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dynan W. S., Tjian R. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. 1985 Aug 29-Sep 4Nature. 316(6031):774–778. doi: 10.1038/316774a0. [DOI] [PubMed] [Google Scholar]
  7. Green P. J., Kay S. A., Chua N. H. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J. 1987 Sep;6(9):2543–2549. doi: 10.1002/j.1460-2075.1987.tb02542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guha A., Bach R., Konigsberg W., Nemerson Y. Affinity purification of human tissue factor: interaction of factor VII and tissue factor in detergent micelles. Proc Natl Acad Sci U S A. 1986 Jan;83(2):299–302. doi: 10.1073/pnas.83.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huang J. K., Swegle M., Dandekar A. M., Muthukrishnan S. Expression and regulation of alpha-amylase gene family in barley aleurones. J Mol Appl Genet. 1984;2(6):579–588. [PubMed] [Google Scholar]
  10. Jofuku K. D., Okamuro J. K., Goldberg R. B. Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature. 1987 Aug 20;328(6132):734–737. doi: 10.1038/328734a0. [DOI] [PubMed] [Google Scholar]
  11. Jones N. C., Rigby P. W., Ziff E. B. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev. 1988 Mar;2(3):267–281. doi: 10.1101/gad.2.3.267. [DOI] [PubMed] [Google Scholar]
  12. Maier U. G., Brown J. W., Tologcyzki C., Feix G. Binding of a nuclear factor to a consensus sequence in the 5' flanking region of zein genes from maize. EMBO J. 1987 Jan;6(1):17–22. doi: 10.1002/j.1460-2075.1987.tb04712.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Paul A. L., Vasil V., Vasil I. K., Ferl R. J. Constitutive and anaerobically induced DNase-I-hypersensitive sites in the 5' region of the maize Adh1 gene. Proc Natl Acad Sci U S A. 1987 Feb;84(3):799–803. doi: 10.1073/pnas.84.3.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reiser H., Coligan J., Palmer E., Benacerraf B., Rock K. L. Cloning and expression of a cDNA for the T-cell-activating protein TAP. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2255–2259. doi: 10.1073/pnas.85.7.2255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rogers J. C. Two barley alpha-amylase gene families are regulated differently in aleurone cells. J Biol Chem. 1985 Mar 25;260(6):3731–3738. [PubMed] [Google Scholar]
  16. Roussell D. L., Boston R. S., Goldsbrough P. B., Larkins B. A. Deletion of DNA sequences flanking an Mr 19,000 zein gene reduces its transcriptional activity in heterologous plant tissues. Mol Gen Genet. 1988 Feb;211(2):202–209. doi: 10.1007/BF00330595. [DOI] [PubMed] [Google Scholar]
  17. Rusconi S., Yamamoto K. R. Functional dissection of the hormone and DNA binding activities of the glucocorticoid receptor. EMBO J. 1987 May;6(5):1309–1315. doi: 10.1002/j.1460-2075.1987.tb02369.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stockhaus J., Eckes P., Rocha-Sosa M., Schell J., Willmitzer L. Analysis of cis-active sequences involved in the leaf-specific expression of a potato gene in transgenic plants. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7943–7947. doi: 10.1073/pnas.84.22.7943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tanaka Y., Ito T., Akazawa T. Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds: III. alpha-Amylase Isozymes. Plant Physiol. 1970 Nov;46(5):650–654. doi: 10.1104/pp.46.5.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walker J. C., Howard E. A., Dennis E. S., Peacock W. J. DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6624–6628. doi: 10.1073/pnas.84.19.6624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wu C., Wilson S., Walker B., Dawid I., Paisley T., Zimarino V., Ueda H. Purification and properties of Drosophila heat shock activator protein. Science. 1987 Nov 27;238(4831):1247–1253. doi: 10.1126/science.3685975. [DOI] [PubMed] [Google Scholar]
  22. Yamamoto K. R. Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet. 1985;19:209–252. doi: 10.1146/annurev.ge.19.120185.001233. [DOI] [PubMed] [Google Scholar]
  23. Zwar J. A., Hooley R. Hormonal Regulation of alpha-Amylase Gene Transcription in Wild Oat (Avena fatua L.) Aleurone Protoplasts. Plant Physiol. 1986 Feb;80(2):459–463. doi: 10.1104/pp.80.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES