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. 1988 Sep;85(17):6399–6403. doi: 10.1073/pnas.85.17.6399

Developmental regulation of embryonic genes in plants

Chumpol Borkird *, Jung H Choi †, Zheng-Hua Jin ‡, Gerald Franz §, Polydefkis Hatzopoulos §, Robert Chorneau §, Ulla Bonas §, Francisco Pelegri , Z Renee Sung §
PMCID: PMC281979  PMID: 16593974

Abstract

Somatic embryogenesis from cultured carrot cells progresses through successive morphogenetic stages termed globular, heart, and torpedo. To understand the molecular mechanisms underlying plant embryogenesis, we isolated two genes differentially expressed during embryo development. The expression of these two genes is associated with heart-stage embryogenesis. By altering the culture conditions and examining their expressions in a developmental variant cell line, we found that these genes were controlled by the developmental program of embryogenesis and were not directly regulated by 2,4-dichlorophenoxyacetic acid, the growth regulator that promotes unorganized growth of cultured cells and suppresses embryo morphogenesis. These genes are also expressed in carrot zygotic embryos but not in seedlings or mature plants.

Keywords: somatic embryogenesis, tissue-specific expression, Daucus carota, embryogenic variant

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Selected References

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