Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Sep;85(18):6783–6787. doi: 10.1073/pnas.85.18.6783

Measurement of outward translocation of phospholipids across human erythrocyte membrane.

M Bitbol 1, P F Devaux 1
PMCID: PMC282062  PMID: 3137572

Abstract

Spin-labeled phospholipids have been used to study the outside----inside and inside----outside transport of phospholipids across the human erythrocyte membrane at 37 degrees C. As already shown, inward transport is much faster for aminophospholipids than for phosphatidylcholine. In addition, we show here that outward transport of the phosphatidylserine and phosphatidylethanolamine analogues is three to four times faster than that of phosphatidylcholine. Magnesium depletion of the erythrocytes considerably decreases the outward rate of both aminophospholipids to values close to that of phosphatidylcholine. These results suggest that the outward aminophospholipid translocation is, at least partly, protein mediated. The protein involved could be identical to the inward Mg-ATP-dependent aminophospholipid carrier.

Full text

PDF
6783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bitbol M., Fellmann P., Zachowski A., Devaux P. F. Ion regulation of phosphatidylserine and phosphatidylethanolamine outside-inside translocation in human erythrocytes. Biochim Biophys Acta. 1987 Nov 13;904(2):268–282. doi: 10.1016/0005-2736(87)90376-2. [DOI] [PubMed] [Google Scholar]
  2. Connor J., Schroit A. J. Determination of lipid asymmetry in human red cells by resonance energy transfer. Biochemistry. 1987 Aug 11;26(16):5099–5105. doi: 10.1021/bi00390a031. [DOI] [PubMed] [Google Scholar]
  3. Daleke D. L., Huestis W. H. Incorporation and translocation of aminophospholipids in human erythrocytes. Biochemistry. 1985 Sep 24;24(20):5406–5416. doi: 10.1021/bi00341a019. [DOI] [PubMed] [Google Scholar]
  4. Herrmann A., Müller P. A model for the asymmetric lipid distribution in the human erythrocyte membrane. Biosci Rep. 1986 Feb;6(2):185–191. doi: 10.1007/BF01115005. [DOI] [PubMed] [Google Scholar]
  5. Martin O. C., Pagano R. E. Transbilayer movement of fluorescent analogs of phosphatidylserine and phosphatidylethanolamine at the plasma membrane of cultured cells. Evidence for a protein-mediated and ATP-dependent process(es). J Biol Chem. 1987 Apr 25;262(12):5890–5898. [PubMed] [Google Scholar]
  6. Seigneuret M., Devaux P. F. ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3751–3755. doi: 10.1073/pnas.81.12.3751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Seigneuret M., Zachowski A., Hermann A., Devaux P. F. Asymmetric lipid fluidity in human erythrocyte membrane: new spin-label evidence. Biochemistry. 1984 Sep 11;23(19):4271–4275. doi: 10.1021/bi00314a002. [DOI] [PubMed] [Google Scholar]
  8. Sune A., Bette-Bobillo P., Bienvenüe A., Fellmann P., Devaux P. F. Selective outside-inside translocation of aminophospholipids in human platelets. Biochemistry. 1987 Jun 2;26(11):2972–2978. doi: 10.1021/bi00385a003. [DOI] [PubMed] [Google Scholar]
  9. Tilley L., Cribier S., Roelofsen B., Op den Kamp J. A., van Deenen L. L. ATP-dependent translocation of amino phospholipids across the human erythrocyte membrane. FEBS Lett. 1986 Jan 1;194(1):21–27. doi: 10.1016/0014-5793(86)80044-8. [DOI] [PubMed] [Google Scholar]
  10. Verkleij A. J., Zwaal R. F., Roelofsen B., Comfurius P., Kastelijn D., van Deenen L. L. The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy. Biochim Biophys Acta. 1973 Oct 11;323(2):178–193. doi: 10.1016/0005-2736(73)90143-0. [DOI] [PubMed] [Google Scholar]
  11. Williamson P., Antia R., Schlegel R. A. Maintenance of membrane phospholipid asymmetry. Lipid-cytoskeletal interactions or lipid pump? FEBS Lett. 1987 Jul 27;219(2):316–320. doi: 10.1016/0014-5793(87)80243-0. [DOI] [PubMed] [Google Scholar]
  12. Zachowski A., Favre E., Cribier S., Hervé P., Devaux P. F. Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzyme. Biochemistry. 1986 May 6;25(9):2585–2590. doi: 10.1021/bi00357a046. [DOI] [PubMed] [Google Scholar]
  13. Zachowski A., Fellman P., Devaux P. F. Absence of transbilayer diffusion of spin-labeled sphingomyelin on human erythrocytes. Comparison with the diffusion of several spin-labeled glycerophospholipids. Biochim Biophys Acta. 1985 May 28;815(3):510–514. doi: 10.1016/0005-2736(85)90380-3. [DOI] [PubMed] [Google Scholar]
  14. Zachowski A., Herrmann A., Paraf A., Devaux P. F. Phospholipid outside-inside translocation in lymphocyte plasma membranes is a protein-mediated phenomenon. Biochim Biophys Acta. 1987 Feb 12;897(1):197–200. doi: 10.1016/0005-2736(87)90328-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES