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. 1988 Sep;85(18):6866–6869. doi: 10.1073/pnas.85.18.6866

Tissue-specific regulatory differences for the alcohol dehydrogenase genes of Hawaiian Drosophila are conserved in Drosophila melanogaster transformants.

M D Brennan 1, C Y Wu 1, A J Berry 1
PMCID: PMC282079  PMID: 3137574

Abstract

Naturally occurring regulatory variation is a source of genetic variability that is well documented but poorly understood. Two members of the Hawaiian picture-winged Drosophila, D. affinidisjuncta and D. hawaiiensis, display markedly different levels of alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) in the larval midgut and Malpighian tubules. To analyze the regulation of the alcohol dehydrogenase genes from these two species, their homologous alcohol dehydrogenase genes were cloned and introduced, via P element-mediated transformation, into the germ line of Drosophila melanogaster. Histochemical and electrophoretic analyses of larval transformants demonstrate that major differences in the tissue-specific levels of alcohol dehydrogenase production are characteristic of the alcohol dehydrogenase genes themselves. While these results do not directly address possible species-specific differences in the tissue distribution of trans-acting regulatory components, they indicate that demonstrable differences in cis-dominant regulatory information are sufficient to account for the observed regulatory variation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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