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. Author manuscript; available in PMC: 2010 Feb 12.
Published in final edited form as: Biochemistry. 2008 Aug 13;47(36):9505. doi: 10.1021/bi8007142

Figure 6. Actin-translocating velocity of USH1B mutants in in vitro motility assay.

Figure 6

The movement of the fluorescent-labeled actin filament was observed in 25 mM KCl, 20 mM MOPS-KOH (pH 7.5), 2 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 36 μg/ml catalase, 4.5 mg/ml glucose, 216 μg/ml glucose oxidase, 0.2 mg/ml calmodulin, and 2 mM MgATP at 25 °C. The error bars indicate S.D. for n=30-40 from three independent preparations.