. Author manuscript; available in PMC: 2010 Feb 12.

Published in final edited form as: Biochemistry. 2008 Aug 13;47(36):9505. doi: 10.1021/bi8007142

Table I.

Steady-state and the ADP release rate constants of human myosin VIIa.

	Ca²⁺ ATPase (s⁻¹)	Actin-activated Mg²⁺ATPase				ADP release rate from actomyosin (s⁻¹)
	Ca²⁺ ATPase (s⁻¹)	v₀ (s⁻¹)	30μM actin (s⁻¹)	V_max (s⁻¹)	K_ATPase (μM)	ADP release rate from actomyosin (s⁻¹)
Wild-type	2.95 ± 0.09	0.05 ± 0.01	0.37 ± 0.04	0.45 ± 0.05	12.8 ± 2.9	0.56 ± 0.05
G25R	0.66 ± 0.12^*	0.08 ± 0.04	0.09 ± 0.04^*	N.D.	N.D.	0.23 ± 0.08^*
R212C	2.00 ± 0.23^*	0.07 ± 0.01	0.07 ± 0.02^*	N.D.	N.D.	0.58 ± 0.12
R302H	2.56 ± 0.03^*	0.05 ± 0.02	0.25 ± 0.02	0.34 ± 0.05	15.2 ± 4.8	0.67 ± 0.14
A397D	2.71 ± 0.09	0.05 ± 0.01	0.07 ± 0.01^*	N.D.	N.D.	0.43 ± 0.12^*
E450Q	1.27 ± 0.08^*	0.04 ± 0.01	0.02 ± 0.01^*	N.D.	N.D.	0.72 ± 0.20
P503L	0.31 ± 0.10^*	0.13 ± 0.01^*	0.23 ± 0.01^*	0.23 ± 0.02^*	3.3 ± 1.6^*	0.79 ± 0.04^*

All date are presented as mean value ± S.D. (n=3 from three independent preparations of each mutant)

P< 0.01 versus wild-type. N.D., Not Detected.