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. 1988 Oct;85(19):7044–7048. doi: 10.1073/pnas.85.19.7044

Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases are single enzymes in Golgi vesicles from rat liver.

G Pohlentz 1, D Klein 1, G Schwarzmann 1, D Schmitz 1, K Sandhoff 1
PMCID: PMC282120  PMID: 3140234

Abstract

Competition experiments using lactosylceramide, ganglioside GM3 and ganglioside GD3 as substrates, as well as mutual inhibitors for ganglioside N-acetylgalactosaminyltransferase, in Golgi vesicles derived from rat liver suggested that N-acetylgalactosamine transfer to these three respective compounds, leading to gangliosides GA2, GM2, and GD2, respectively, is catalyzed by one enzyme. Analogous studies with gangliosides GA1, GM1, and GD1b as glycolipid acceptors in sialyltransferase assays indicated GM1b, GD1a, and GT1b synthases to be identical. These results are incorporated into a model for ganglioside biosynthesis and its regulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basu M., De T., Das K. K., Kyle J. W., Chon H. C., Schaeper R. J., Basu S. Glycolipids. Methods Enzymol. 1987;138:575–607. doi: 10.1016/0076-6879(87)38053-x. [DOI] [PubMed] [Google Scholar]
  2. Busam K., Decker K. Ganglioside biosynthesis in rat liver. Characterization of three sialyltransferases. Eur J Biochem. 1986 Oct 1;160(1):23–30. doi: 10.1111/j.1432-1033.1986.tb09934.x. [DOI] [PubMed] [Google Scholar]
  3. Cumar F. A., Tallman J. F., Brady R. O. The biosynthesis of a disialylganglioside by galactosyltransferase from rat brain tissue. J Biol Chem. 1972 Apr 25;247(8):2322–2327. [PubMed] [Google Scholar]
  4. Derry D. M., Wolfe L. S. Gangliosides in isolated neurons and glial cells. Science. 1967 Dec 15;158(3807):1450–1452. doi: 10.1126/science.158.3807.1450. [DOI] [PubMed] [Google Scholar]
  5. Eppler C. M., Morré D. J., Keenan T. W. Ganglioside biosynthesis in rat liver: characterization of cytidine-5'-monophospho-n-acetylneuraminic acid:hematoside (GM3) sialyltransferase. Biochim Biophys Acta. 1980 Aug 11;619(2):318–331. doi: 10.1016/0005-2760(80)90080-6. [DOI] [PubMed] [Google Scholar]
  6. Fishman P. H., Max S. R., Tallman J. F., Brady R. O., Maclaren N. K., Cornblath M. Deficient Ganglioside Biosynthesis: a novel human sphingolipidosis. Science. 1975 Jan 10;187(4171):68–70. doi: 10.1126/science.803227. [DOI] [PubMed] [Google Scholar]
  7. Hamberger A., Svennerholm L. Composition of gangliosides and phospholipids of neuronal and glial cell enriched fractions. J Neurochem. 1971 Oct;18(10):1821–1829. doi: 10.1111/j.1471-4159.1971.tb09587.x. [DOI] [PubMed] [Google Scholar]
  8. Kaplan F., Hechtman P. Purification and properties of two enzymes catalyzing galactose transfer to GM2 ganglioside from rat liver Golgi. J Biol Chem. 1983 Jan 25;258(2):770–776. [PubMed] [Google Scholar]
  9. Keenan T. W., Doak R. L. Enzymatic block in higher ganglioside biosynthesis in avian transplantable lymphoid tumor. FEBS Lett. 1973 Dec 1;37(2):124–128. doi: 10.1016/0014-5793(73)80440-5. [DOI] [PubMed] [Google Scholar]
  10. Keenan T. W., Morré D. J., Basu S. Ganglioside biosynthesis. Concentration of glycosphingolipid glycosyltransferases in Golgi apparatus from rat liver. J Biol Chem. 1974 Jan 10;249(1):310–315. [PubMed] [Google Scholar]
  11. Klein D., Pohlentz G., Schwarzmann G., Sandhoff K. Substrate specificity of GM2 and GD3 synthase of Golgi vesicles derived from rat liver. Eur J Biochem. 1987 Sep 15;167(3):417–424. doi: 10.1111/j.1432-1033.1987.tb13354.x. [DOI] [PubMed] [Google Scholar]
  12. LOWDEN J. A., WOLFE L. S. STUDIES ON BRAIN GANGLIOSIDES. 3. EVIDENCE FOR THE LOCATION OF GANGLIOSIDES SPECIFICALLY IN NEURONES. Can J Biochem. 1964 Nov;42:1587–1594. doi: 10.1139/o64-170. [DOI] [PubMed] [Google Scholar]
  13. Ledeen R., Salsman K., Cabrera M. Gangliosides of bovine adrenal medulla. Biochemistry. 1968 Jun;7(6):2287–2295. doi: 10.1021/bi00846a035. [DOI] [PubMed] [Google Scholar]
  14. Pacuszka T., Duffard R. O., Nishimura R. N., Brady R. O., Fishman P. H. Biosynthesis of bovine thyroid gangliosides. J Biol Chem. 1978 Aug 25;253(16):5839–5846. [PubMed] [Google Scholar]
  15. Pohlentz G., Klein D., Schmitz D., Schwarzmann G., Peter-Katalinić J., Sandhoff K. Biosynthesis of gangliosides from asialogangliosides in rat liver Golgi vesicles. Biol Chem Hoppe Seyler. 1988 Jan;369(1):55–63. doi: 10.1515/bchm3.1988.369.1.55. [DOI] [PubMed] [Google Scholar]
  16. Puro K., Maury P., Huttunen J. K. Qualitative and quantitative patterns of gangliosides in extraneural tissues. Biochim Biophys Acta. 1969;187(2):230–235. doi: 10.1016/0005-2760(69)90032-0. [DOI] [PubMed] [Google Scholar]
  17. Richardson C. L., Keenan T. W., Morre D. J. Ganglioside biosynthesis. Characterization of CMP-N-acetylneuraminic acid : lactosylceramide sialyltransferase in Golgi apparatus from rat liver. Biochim Biophys Acta. 1977 Jul 20;488(1):88–96. doi: 10.1016/0005-2760(77)90125-4. [DOI] [PubMed] [Google Scholar]
  18. SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. doi: 10.1111/j.1471-4159.1963.tb08933.x. [DOI] [PubMed] [Google Scholar]
  19. Sandberg P. O., Marzella L., Glaumann H. A method for rapid isolation of rough and smooth microsomes and Golgi apparatus from rat liver in the same sucrose gradient. Exp Cell Res. 1980 Dec;130(2):393–400. doi: 10.1016/0014-4827(80)90017-8. [DOI] [PubMed] [Google Scholar]
  20. Scheel G., Acevedo E., Conzelmann E., Nehrkorn H., Sandhoff K. Model for the interaction of membrane-bound substrates and enzymes. Hydrolysis of ganglioside GD1a by sialidase of neuronal membranes isolated from calf brain. Eur J Biochem. 1982 Oct;127(2):245–253. doi: 10.1111/j.1432-1033.1982.tb06862.x. [DOI] [PubMed] [Google Scholar]
  21. Senn H. J., Cooper C., Warnke P. C., Wagner M., Decker K. Ganglioside biosynthesis in rat liver. Characterization of UDP-N-acetylgalactosamine -- GM3 acetylgalactosaminyltransferase. Eur J Biochem. 1981 Nov;120(1):59–67. doi: 10.1111/j.1432-1033.1981.tb05670.x. [DOI] [PubMed] [Google Scholar]
  22. Senn H. J., Wagner M., Decker K. Ganglioside biosynthesis in rat liver. Characterization of UDPgalactose--glucosylceramide galactosyltransferase and UDPgalactose-GM2 galactosyltransferase. Eur J Biochem. 1983 Sep 15;135(2):231–236. doi: 10.1111/j.1432-1033.1983.tb07642.x. [DOI] [PubMed] [Google Scholar]
  23. Sonderfeld S., Conzelmann E., Schwarzmann G., Burg J., Hinrichs U., Sandhoff K. Incorporation and metabolism of ganglioside GM2 in skin fibroblasts from normal and GM2 gangliosidosis subjects. Eur J Biochem. 1985 Jun 3;149(2):247–255. doi: 10.1111/j.1432-1033.1985.tb08919.x. [DOI] [PubMed] [Google Scholar]
  24. Taki T., Kamada R., Matsumoto M. Glycosphingolipid biosynthesis through asialogangliosides in rat bone marrow cells. J Biochem. 1982 Feb;91(2):665–674. doi: 10.1093/oxfordjournals.jbchem.a133739. [DOI] [PubMed] [Google Scholar]
  25. Wiegandt H. Gangliosides of extraneural organs. Hoppe Seylers Z Physiol Chem. 1973 Sep;354(9):1049–1056. doi: 10.1515/bchm2.1973.354.2.1049. [DOI] [PubMed] [Google Scholar]
  26. Wilkinson F. E., Morré D. J., Keenan T. W. Ganglioside biosynthesis. Characterization of uridine diphosphate galactose: GM2 galactosyltransferase in golgi apparatus from rat liver. J Lipid Res. 1976 Mar;17(2):146–153. [PubMed] [Google Scholar]
  27. Yanagisawa K., Taniguchi N., Makita A. Purification and properties of GM2 synthase, UDP-N-acetylgalactosamine: GM3 beta-N-acetylgalactosaminyltransferase from rat liver. Biochim Biophys Acta. 1987 Jun 23;919(3):213–220. doi: 10.1016/0005-2760(87)90260-8. [DOI] [PubMed] [Google Scholar]
  28. Yusuf H. K., Pohlentz G., Sandhoff K. Tunicamycin inhibits ganglioside biosynthesis in rat liver Golgi apparatus by blocking sugar nucleotide transport across the membrane vesicles. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7075–7079. doi: 10.1073/pnas.80.23.7075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yusuf H. K., Pohlentz G., Schwarzmann G., Sandhoff K. Ganglioside biosynthesis in Golgi apparatus of rat liver. Stimulation by phosphatidylglycerol and inhibition by tunicamycin. Eur J Biochem. 1983 Jul 15;134(1):47–54. doi: 10.1111/j.1432-1033.1983.tb07529.x. [DOI] [PubMed] [Google Scholar]
  30. Yusuf H. K., Schwarzmann G., Pohlentz G., Sandhoff K. Oligosialogangliosides inhibit GM2- and GD3-synthesis in isolated Golgi vesicles from rat liver. Biol Chem Hoppe Seyler. 1987 May;368(5):455–462. doi: 10.1515/bchm3.1987.368.1.455. [DOI] [PubMed] [Google Scholar]

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