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. 1988 Oct;85(19):7159–7163. doi: 10.1073/pnas.85.19.7159

Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

S Cotecchia 1, D A Schwinn 1, R R Randall 1, R J Lefkowitz 1, M G Caron 1, B K Kobilka 1
PMCID: PMC282143  PMID: 2845398

Abstract

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

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Selected References

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