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. Author manuscript; available in PMC: 2011 Feb 10.
Published in final edited form as: Vaccine. 2009 Dec 8;28(6):1454. doi: 10.1016/j.vaccine.2009.11.073

Figure 3. Splenic CD4+ T cells from L2R immunized mice produce a dominant Th1 biased cytokine response.

Figure 3

The in vitro production of cytokines by splenic CD4+ T cells from sham or L2R immunized mice was assayed two weeks following the resolution of the 2° L2R infection. The CD4+ T cells were re-stimulated in vitro with UV-inactivated serovar D EB and the supernatants were collected after 72 h of culture for determining the cytokine concentration. The black bars represent the mean ± standard deviation of four individual L2R treated mice tested in quadruplicate. The white bars represent the results of two individual sham infected mice and expressed as the mean ± standard deviation. There was a significant production of IFN-γ by chlamydial pulsed CD4+ T cells.