Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Oct;85(20):7724–7728. doi: 10.1073/pnas.85.20.7724

A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell.

V M Sanders 1, R Fernandez-Botran 1, R L Coffman 1, T R Mosmann 1, E S Vitetta 1
PMCID: PMC282265  PMID: 2459715

Abstract

Keyhole-limpet-hemocyanin-specific I-Ad-restricted T helper cells type 1 (TH1 cells) and type 2 (TH2 cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (TH) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of TH cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other TH cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of TH cell. A similar pattern of inhibition of the conjugation of one TH cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one TH cell prevents the simultaneous binding of another TH cell.

Full text

PDF
7724

Images in this article

7725Image
on p.7725

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asano Y., Hodes R. J. T cell regulation of b cell activation. Cloned Lyt-1+2-T suppressor cells inhibit the major histocompatibility complex-restricted interaction of T helper cells with B cells and/or accessory cells. J Exp Med. 1983 Oct 1;158(4):1178–1190. doi: 10.1084/jem.158.4.1178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cherwinski H. M., Schumacher J. H., Brown K. D., Mosmann T. R. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987 Nov 1;166(5):1229–1244. doi: 10.1084/jem.166.5.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fernandez-Botran R., Sanders V. M., Oliver K. G., Chen Y. W., Krammer P. H., Uhr J. W., Vitetta E. S. Interleukin 4 mediates autocrine growth of helper T cells after antigenic stimulation. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9689–9693. doi: 10.1073/pnas.83.24.9689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forman J., Ciavarra R., Vitetta E. S. Cytotoxic T cells specific for antigens expressed on surface immunoglobulin-positive cells. J Exp Med. 1981 Nov 1;154(5):1357–1368. doi: 10.1084/jem.154.5.1357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Katz D. H., Graves M., Dorf M. E., Dimuzio H., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex. J Exp Med. 1975 Jan 1;141(1):263–268. doi: 10.1084/jem.141.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kim J., Woods A., Becker-Dunn E., Bottomly K. Distinct functional phenotypes of cloned Ia-restricted helper T cells. J Exp Med. 1985 Jul 1;162(1):188–201. doi: 10.1084/jem.162.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kupfer A., Singer S. J., Janeway C. A., Jr, Swain S. L. Coclustering of CD4 (L3T4) molecule with the T-cell receptor is induced by specific direct interaction of helper T cells and antigen-presenting cells. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5888–5892. doi: 10.1073/pnas.84.16.5888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kupfer A., Swain S. L., Janeway C. A., Jr, Singer S. J. The specific direct interaction of helper T cells and antigen-presenting B cells. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6080–6083. doi: 10.1073/pnas.83.16.6080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marrack P., Kappler J. W. The role of H-2-linked genes in helper T cell function. VII. Expression of I region and immune response genes by B cells in bystander help assays. J Exp Med. 1980 Nov 1;152(5):1274–1288. doi: 10.1084/jem.152.5.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. II. Cellular cooperation. Eur J Immunol. 1971 Jan;1(1):18–27. doi: 10.1002/eji.1830010104. [DOI] [PubMed] [Google Scholar]
  11. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  12. Myers C. D., Sanders V. M., Vitetta E. S. Isolation of antigen-binding virgin and memory B cells. J Immunol Methods. 1986 Aug 21;92(1):45–57. doi: 10.1016/0022-1759(86)90502-8. [DOI] [PubMed] [Google Scholar]
  13. Noelle R. J., Snow E. C., Uhr J. W., Vitetta E. S. Activation of antigen-specific B cells: role of T cells, cytokines, and antigen in induction of growth and differentiation. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6628–6631. doi: 10.1073/pnas.80.21.6628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rajewsky K., Schirrmacher V., Nase S., Jerne N. K. The requirement of more than one antigenic determinant for immunogenicity. J Exp Med. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanders V. M., Snyder J. M., Uhr J. W., Vitetta E. S. Characterization of the physical interaction between antigen-specific B and T cells. J Immunol. 1986 Oct 15;137(8):2395–2404. [PubMed] [Google Scholar]
  16. Sheil J. M., Bevan M. J., Lefrancois L. Characterization of dual-reactive H-2Kb-restricted anti-vesicular stomatitus virus and alloreactive cytotoxic T cells. J Immunol. 1987 Jun 1;138(11):3654–3660. [PubMed] [Google Scholar]
  17. Shimonkevitz R., Kappler J., Marrack P., Grey H. Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. J Exp Med. 1983 Aug 1;158(2):303–316. doi: 10.1084/jem.158.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Snow E. C., Vitetta E. S., Uhr J. W. Activation of antigen-enriched b cells. I. Purification and response to thymus-independent antigens. J Immunol. 1983 Feb;130(2):607–613. [PubMed] [Google Scholar]
  19. Sprent J. Restricted helper function of F1 hybrid T cells positively selected to heterologous erythrocytes in irradiated parental strain mice. I. Failure to collaborate with B cells of the opposite parental strain not associated with active suppression. J Exp Med. 1978 Apr 1;147(4):1142–1158. doi: 10.1084/jem.147.4.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stevens T. L., Bossie A., Sanders V. M., Fernandez-Botran R., Coffman R. L., Mosmann T. R., Vitetta E. S. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature. 1988 Jul 21;334(6179):255–258. doi: 10.1038/334255a0. [DOI] [PubMed] [Google Scholar]
  21. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES