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. 1988 Nov;85(21):7937–7941. doi: 10.1073/pnas.85.21.7937

Purified hepatocyte nuclear factor 1 interacts with a family of hepatocyte-specific promoters.

G Courtois 1, S Baumhueter 1, G R Crabtree 1
PMCID: PMC282328  PMID: 2460858

Abstract

During development cell types arise through the activation or repression of classes of specific genes. One hypothesis is that this phenomenon is realized by tissue-specific factors playing a role at the transcription level. Recently we have described a liver-specific nuclear protein, hepatocyte nuclear factor 1, that appears to be involved in the transcription of the fibrinogen and alpha 1-antitrypsin genes. In this report we describe the purification of hepatocyte nuclear factor 1 and demonstrate that it interacts with essential promoter regions of many liver-specific genes, including albumin, alpha-fetoprotein, and transthyretin. This finding suggests that hepatocyte nuclear factor 1 could be one factor necessary for establishing the liver phenotype. We also show that this protein binds to the promoter of the surface-antigen gene of the hepatitis B virus, a virus characterized by a high degree of hepatotropism.

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Selected References

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