Figure 2. Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities.
a, Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p<0.02, compared with GST, n=3. c, d, The interaction of TLS N (1-211):C (373-526) termini (c) or GST-TLS:p300 (d) in the presence of GGUG- or CCUC-oligonucleotide. GST and GST-TLS were pre-treated with RNase A. Error bars indicate ± SEM.