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. 1988 Nov;85(21):8241–8245. doi: 10.1073/pnas.85.21.8241

Expression of the human growth hormone variant gene in cultured fibroblasts and transgenic mice.

R F Selden 1, T E Wagner 1, S Blethen 1, J S Yun 1, M E Rowe 1, H M Goodman 1
PMCID: PMC282405  PMID: 3186721

Abstract

The nucleotide sequence of the human growth hormone variant gene, one of the five members of the growth hormone gene family, predicts that it encodes a growth hormone-like protein. As a first step in determining whether this gene is functional in humans, we have expressed a mouse metallothionein I/human growth hormone variant fusion gene in mouse L cells and in transgenic mice. The growth hormone variant protein expressed in transiently transfected L cells is distinct from growth hormone itself with respect to reactivity with anti-growth hormone monoclonal antibodies, behavior during column chromatography, and isoelectric point. Transgenic mice expressing the growth hormone variant protein are 1.4- to 1.9-fold larger than nontransgenic controls, suggesting that the protein has growth-promoting properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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