Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Nov;85(22):8410–8414. doi: 10.1073/pnas.85.22.8410

Repair of 4,5',8-trimethylpsoralen monoadducts and cross-links by the Escherichia coli UvrABC endonuclease.

B K Jones 1, A T Yeung 1
PMCID: PMC282467  PMID: 3054888

Abstract

Using an oligonucleotide model substrate, we observed two unusual mechanisms of UvrABC endonuclease in the repair of 4,5',8-trimethylpsoralen monoadducts and crosslinks. (i) UvrABC endonuclease usually incises a psoralen monoadduct only on the damaged strand. However, for one of the monoadducts we studied, incision on the complementary undamaged strand was also observed at a very low frequency, as though the adduct were on the thymine across from the damaged strand. Although the details of the erroneous incision are not yet known, such erroneous incision is potentially mutagenic. (ii) In cross-link repair, we observed that the UvrABC endonuclease incises the cross-linked DNA on either the furan side strand or the pyrone side strand. The incisions are not equally efficient. These data suggest that the structure of a psoralen cross-link, as seen by a repair enzyme, varies with the DNA sequence.

Full text

PDF

Images in this article

8412Image
on p.8412
8412Image
on p.8412
8413Image
on p.8413
8413Image
on p.8413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cimino G. D., Gamper H. B., Isaacs S. T., Hearst J. E. Psoralens as photoactive probes of nucleic acid structure and function: organic chemistry, photochemistry, and biochemistry. Annu Rev Biochem. 1985;54:1151–1193. doi: 10.1146/annurev.bi.54.070185.005443. [DOI] [PubMed] [Google Scholar]
  2. Cole R. S., Levitan D., Sinden R. R. Removal of psoralen interstrand cross-links from DNA of Escherichia coli: mechanism and genetic control. J Mol Biol. 1976 May 5;103(1):39–59. doi: 10.1016/0022-2836(76)90051-6. [DOI] [PubMed] [Google Scholar]
  3. Friedberg E. C., Backendorf C., Burke J., Collins A., Grossman L., Hoeijmakers J. H., Lehmann A. R., Seeberg E., van der Schans G. P., van Zeeland A. A. Molecular aspects of DNA repair. Mutat Res. 1987 Sep;184(2):67–86. doi: 10.1016/0167-8817(87)90063-0. [DOI] [PubMed] [Google Scholar]
  4. Howard-Flanders P. DNA repair and recombination. Br Med Bull. 1973 Sep;29(3):226–235. doi: 10.1093/oxfordjournals.bmb.a071012. [DOI] [PubMed] [Google Scholar]
  5. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  6. Sancar A., Franklin K. A., Sancar G., Tang M. S. Repair of psoralen and acetylaminofluorene DNA adducts by ABC excinuclease. J Mol Biol. 1985 Aug 20;184(4):725–734. doi: 10.1016/0022-2836(85)90316-x. [DOI] [PubMed] [Google Scholar]
  7. Scott B. R., Pathak M. A., Mohn G. R. Molecular and genetic basis of furocoumarin reactions. Mutat Res. 1976;39(1):29–74. doi: 10.1016/0165-1110(76)90012-9. [DOI] [PubMed] [Google Scholar]
  8. Tessman J. W., Isaacs S. T., Hearst J. E. Photochemistry of the furan-side 8-methoxypsoralen-thymidine monoadduct inside the DNA helix. Conversion to diadduct and to pyrone-side monoadduct. Biochemistry. 1985 Mar 26;24(7):1669–1676. doi: 10.1021/bi00328a015. [DOI] [PubMed] [Google Scholar]
  9. Van Houten B., Gamper H., Holbrook S. R., Hearst J. E., Sancar A. Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8077–8081. doi: 10.1073/pnas.83.21.8077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  11. Yeung A. T., Jones B. K., Capraro M., Chu T. The repair of psoralen monoadducts by the Escherichia coli UvrABC endonuclease. Nucleic Acids Res. 1987 Jun 25;15(12):4957–4971. doi: 10.1093/nar/15.12.4957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yeung A. T., Jones B. K., Chu C. T. Photoreactivities and thermal properties of psoralen cross-links. Biochemistry. 1988 May 3;27(9):3204–3210. doi: 10.1021/bi00409a012. [DOI] [PubMed] [Google Scholar]
  13. Yeung A. T., Mattes W. B., Grossman L. Protein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease. Nucleic Acids Res. 1986 Mar 25;14(6):2567–2582. doi: 10.1093/nar/14.6.2567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yeung A. T., Mattes W. B., Oh E. Y., Grossman L. Enzymatic properties of purified Escherichia coli uvrABC proteins. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6157–6161. doi: 10.1073/pnas.80.20.6157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yeung A. T., Mattes W. B., Oh E. Y., Yoakum G. H., Grossman L. The purification of the Escherichia coli UvrABC incision system. Nucleic Acids Res. 1986 Nov 11;14(21):8535–8556. doi: 10.1093/nar/14.21.8535. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES