Cytokine responses in BCG vaccinated and unvaccinated infants. Cytokines in supernatants from diluted whole blood cultures which were stimulated with M.tb PPD for 6 days were measured using a Multiplex assay in infants who had been BCG vaccinated 3 months previously and in unvaccinated control infants. Pro-inflammatory cytokines IL-1α, IL-2, IL-6, TNFα and IFNγ, TH17 cytokine IL-17, TH2 cytokines IL-4, IL-5, IL-13, chemokines IL-8, IP-10, MIP-1α and growth factors G-CSF, GM-CSF.