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. 1988 Dec;85(24):9660–9663. doi: 10.1073/pnas.85.24.9660

Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.

A S Cohen 1, D R Najarian 1, A Paulus 1, A Guttman 1, J A Smith 1, B L Karger 1
PMCID: PMC282829  PMID: 3200850

Abstract

Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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