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. 1970 Feb;65(2):304–309. doi: 10.1073/pnas.65.2.304

Retention of Viral Antigen in the Cytoplasm of Cells Infected with Temperature-Sensitive Mutants of an Avian Adenovirus

Masahide Ishibashi 1
PMCID: PMC282902  PMID: 4905673

Abstract

Immunofluorescent studies were made on chicken kidney cell cultures infected with various temperature-sensitive (ts) mutants of an avian adenovirus, chicken embryo lethal orphan (CELO), which grow well at the nonrestrictive temperature of 31°, but fail to grow at the restrictive temperature of 40°. At 40° some mutants (ts 5, 8, and 10) accumulated viral antigen in the cytoplasm, but scarcely at all in the nucleus. However, at 31° they accumulated the antigen in the nucleus like the wild-type strain at either 31° or 40°. These mutants seemed to complement each other in nuclear accumulation of antigen at 40°. In cells infected with ts 8, the antigen remaining in the cytoplasm during incubation at 40° was partially transferred to the nucleus even in the presence of an inhibitor of protein synthesis when the temperature was lowered to 31°. The results suggest that viral antigen produced in the cytoplasm is transported to the nucleus and that these ts mutants are defective in this process at 40°.

There are apparently two other types of ts mutant: one type (ts 3, 7, and 12), like the wild-type strain, showing nuclear accumulation of antigen at 40° and the other (ts 6, 11, and 17) showing no, or only slight, formation of the antigen at 40°.

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