Abstract
Isolated segments of renal tubules prepared from rat kidney cortex were capable of sustained rates of gluconeogenesis when lactate served as substrate. An increase in external Ca++, or the addition of parathyroid hormone at a fixed Ca++ concentration led to enhanced rate of gluconeogenesis. However, parathyroid did not increase gluconeogenesis in the absence of external Ca++ even though it caused a rise in 3′5′ AMP whether Ca++ was present or absent. Metabolite profiles showed that either an increase in external Ca++, or the addition of hormone in the presence of calcium led to qualitatively very similar changes. However, the addition of hormone had little effect upon metabolite levels in the absence of extracellular calcium even though an increase in extracellular H+ enhanced gluconeogenesis under similar conditions. These results are discussed in relationship to the close association of 3′5′ AMP and Ca++ in a variety of cellular systems.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borle A. B. Calcium metabolism in HeLa cells and the effects of parathyroid hormone. J Cell Biol. 1968 Mar;36(3):567–582. doi: 10.1083/jcb.36.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borle A. B. Effects of purified parathyroid hormone on the calcium metabolism of monkey kidney cells. Endocrinology. 1968 Dec;83(6):1316–1322. doi: 10.1210/endo-83-6-1316. [DOI] [PubMed] [Google Scholar]
- Castañeda M., Tyler A. Adenyl cyclase in plasma membrane preparations of sea urchin eggs and its increase in activity after fertilization. Biochem Biophys Res Commun. 1968 Dec 9;33(5):782–787. doi: 10.1016/0006-291x(68)90228-3. [DOI] [PubMed] [Google Scholar]
- Chase L. R., Aurbach G. D. Parathyroid function and the renal excretion of 3'5'-adenylic acid. Proc Natl Acad Sci U S A. 1967 Aug;58(2):518–525. doi: 10.1073/pnas.58.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase L. R., Aurbach G. D. Renal adenyl cyclase: anatomically separate sites for parathyroid hormone and vasopressin. Science. 1968 Feb 2;159(3814):545–547. doi: 10.1126/science.159.3814.545. [DOI] [PubMed] [Google Scholar]
- Chase L. R., Fedak S. A., Aurbach G. D. Activation of skeletal adenyl cyclase by parathyroid hormone in vitro. Endocrinology. 1969 Apr;84(4):761–768. doi: 10.1210/endo-84-4-761. [DOI] [PubMed] [Google Scholar]
- Friedmann N., Park C. R. Early effects of 3',5'-adenosine monophosphate on the fluxes of calcium end potassium in the perfused liver of normal and adrenalectomized rats. Proc Natl Acad Sci U S A. 1968 Oct;61(2):504–508. doi: 10.1073/pnas.61.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gingell D., Garrod D. R. Effect of EDTA on electrophoretic mobility of slime mould cells and its relationship to current theories of cell adhesion. Nature. 1969 Jan 11;221(5176):192–193. doi: 10.1038/221192a0. [DOI] [PubMed] [Google Scholar]
- Goldberg N. D., Larner J., Sasko H., O'Toole A. G. Enzymic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine. Anal Biochem. 1969 Apr 4;28(1):523–544. doi: 10.1016/0003-2697(69)90208-5. [DOI] [PubMed] [Google Scholar]
- Goodman A. D., Fuisz R. E., Cahill G. F., Jr Renal gluconeogenesis in acidosis, alkalosis, and potassium deficiency: its possible role in regulation of renal ammonia production. J Clin Invest. 1966 Apr;45(4):612–619. doi: 10.1172/JCI105375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawker C. D., Glass J. D., Rasmussen H. Further studies on the isolation and charcterization of parathyroid polypeptides. Biochemistry. 1966 Jan;5(1):344–352. doi: 10.1021/bi00865a044. [DOI] [PubMed] [Google Scholar]
- Howard R. B., Pesch L. A. Respiratory activity of intact, isolated parenchymal cells from rat liver. J Biol Chem. 1968 Jun 10;243(11):3105–3109. [PubMed] [Google Scholar]
- KREBS H. A., BENNETT D. A., DE GASQUET P., GASQUET P., GASCOYNE T., YOSHIDA T. Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices. Biochem J. 1963 Jan;86:22–27. doi: 10.1042/bj0860022. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAITRA P. K., ESTABROOK R. W. A FLUOROMETRIC METHOD FOR THE ENZYMIC DETERMINATION OF GLYCOLYTIC INTERMEDIATES. Anal Biochem. 1964 Apr;7:472–484. doi: 10.1016/0003-2697(64)90156-3. [DOI] [PubMed] [Google Scholar]
- Nagata N., Rasmussen H. Parathyroid hormone and renal cell metabolism. Biochemistry. 1968 Oct;7(10):3728–3733. doi: 10.1021/bi00850a053. [DOI] [PubMed] [Google Scholar]
- Namm D. H., Mayer S. E., Maltbie M. The role of potassium and calcium ions in the effect of epinephrine on cardiac cyclic adenosine 3',5'-monophosphate, phosphorylase kinase, and phosphorylase. Mol Pharmacol. 1968 Sep;4(5):522–530. [PubMed] [Google Scholar]
- Rasmussen H., Pechet M., Fast D. Effect of dibutyryl cyclic adenosine 3',5'-monophosphate, theophylline, and other nucleotides upon calcium and phosphate metabolism. J Clin Invest. 1968 Aug;47(8):1843–1850. doi: 10.1172/JCI105874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rasmussen H., Tenenhouse A. Cyclic adenosine monophosphate, CA++, and membranes. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1364–1370. doi: 10.1073/pnas.59.4.1364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUTHERLAND E. W., OYE I., BUTCHER R. W. THE ACTION OF EPINEPHRINE AND THE ROLE OF THE ADENYL CYCLASE SYSTEM IN HORMONE ACTION. Recent Prog Horm Res. 1965;21:623–646. [PubMed] [Google Scholar]
- Samli M. H., Geschwind I. I. Some effects of energy-transfer inhibitors and of Ca++-free or K+-enhanced media on the release of luteinizing hormone (LH) from the rat pituitary gland in vitro. Endocrinology. 1968 Feb;82(2):225–231. doi: 10.1210/endo-82-2-225. [DOI] [PubMed] [Google Scholar]
- Simpson D. P., Sherrard D. J. Regulation of glutamine metabolism in vitro by bicarbonate ion and pH. J Clin Invest. 1969 Jun;48(6):1088–1096. doi: 10.1172/JCI106065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vale W., Burgus R., Guillemin R. Presence of calcium ions as a requisite for the in vitro stimulation of TSH-release by hypothalamic TRF. Experientia. 1967 Oct 15;23(10):853–855. doi: 10.1007/BF02146887. [DOI] [PubMed] [Google Scholar]
- Zor U., Lowe I. P., Bloom G., Field J. B. The role of calcium (Ca++) in TSH and dibutyryl 3'5' cyclic AMP stimulation of thyroid glucose oxidation and phospholipid synthesis. Biochem Biophys Res Commun. 1968 Nov 25;33(4):649–658. doi: 10.1016/0006-291x(68)90345-8. [DOI] [PubMed] [Google Scholar]