Abstract
The average interval between attachment of a 30S ribosomal subunit to bacteriophage T4 lysozyme mRNA and the completion of synthesis of lysozyme protein has been measured in vitro. The measured completion times yield rates of polypeptide chain propagation of 1.6 and 3.0 amino acids per second at 25°C and 31°C, respectively.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA. J Mol Biol. 1968 Feb 14;31(3):325–348. doi: 10.1016/0022-2836(68)90413-0. [DOI] [PubMed] [Google Scholar]
- Bremer H., Yuan D. Chain growth rate of messenger RNA in Escherichia coli infected with bacteriophage T4. J Mol Biol. 1968 Jun 28;34(3):527–540. doi: 10.1016/0022-2836(68)90178-2. [DOI] [PubMed] [Google Scholar]
- Capecchi M. R. Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins. J Mol Biol. 1966 Oct 28;21(1):173–193. doi: 10.1016/0022-2836(66)90086-6. [DOI] [PubMed] [Google Scholar]
- Darlix J. L., Sentenac A., Fromageot P. Etude du RNA synthétisé in vitro. Biochim Biophys Acta. 1968 Sep 24;166(2):438–458. [PubMed] [Google Scholar]
- Gold L. M., Schweiger M. Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro. Proc Natl Acad Sci U S A. 1969 Mar;62(3):892–898. doi: 10.1073/pnas.62.3.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grollman A. P., Stewart M. L. Inhibition of the attachment of messenger ribonucleic acid to ribosomes. Proc Natl Acad Sci U S A. 1968 Oct;61(2):719–725. doi: 10.1073/pnas.61.2.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
- LEDER P., NIRENBERG M. RNA CODEWORDS AND PROTEIN SYNTHESIS. II. NUCLEOTIDE SEQUENCE OF A VALINE RNA CODEWORD. Proc Natl Acad Sci U S A. 1964 Aug;52:420–427. doi: 10.1073/pnas.52.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lacroute F., Stent G. S. Peptide chain growth of -galactosidase in Escherichia coli. J Mol Biol. 1968 Jul 14;35(1):165–173. doi: 10.1016/s0022-2836(68)80044-0. [DOI] [PubMed] [Google Scholar]
- Mangiarotti G., Schlessinger D. Polyribosome metabolism in Escherichia coli. I. Extraction of polyribosomes and ribosomal subunits from fragile, growing Escherichia coli. J Mol Biol. 1966 Sep;20(1):123–143. doi: 10.1016/0022-2836(66)90122-7. [DOI] [PubMed] [Google Scholar]
- Manor H., Goodman D., Stent G. S. RNA chain growth rates in Escherichia coli. J Mol Biol. 1969 Jan 14;39(1):1–29. doi: 10.1016/0022-2836(69)90329-5. [DOI] [PubMed] [Google Scholar]
- Michaelis G., Starlinger P. Sequential appearance of the galactose enzymes in E. coli. Mol Gen Genet. 1967;100(2):210–215. doi: 10.1007/BF00333607. [DOI] [PubMed] [Google Scholar]
- Modolell J., Davis B. D. Rapid inhibition of polypeptide chain extension by streptomycin. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1279–1286. doi: 10.1073/pnas.61.4.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEKIGUCHI M., COHEN S. S. THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS. II. SYNTHESIS OF PHAGE-INDUCED RNA AND SEQUENTIAL ENZYME PRODUCTION. J Mol Biol. 1964 May;8:638–659. doi: 10.1016/s0022-2836(64)80114-5. [DOI] [PubMed] [Google Scholar]
- Stent G. S. Genetic transcription. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):181–197. doi: 10.1098/rspb.1966.0022. [DOI] [PubMed] [Google Scholar]