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. 1970 Mar;65(3):528–535. doi: 10.1073/pnas.65.3.528

Neutralization of Sensitized Virus by Purified Components of Complement*,†

Charles A Daniels 1,2, Tibor Borsos 1,2, Herbert J Rapp 1,2, Ralph Snyderman 1,2, Abner Louis Notkins 1,2
PMCID: PMC282939  PMID: 4315612

Abstract

Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the functionally purified components of C showed that the activated first component of C (C1[unk]) failed to neutralize sensitized virus. However, in the presence of an optimal concentration of C1[unk], the addition of C4 resulted in neutralization. The amount of virus neutralized was dependent upon the concentration of immunoglobulin M used to sensitize the virus and the concentration of C1[unk] and C4. The addition of the second component of C (C2) to reaction mixtures containing an optimal concentration of C1[unk] and a limiting concentration of C4 resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C2. The addition of the third component of C (C3) to reaction mixtures containing an optimal concentration of C1[unk] and limiting concentrations of C4 and C2 also resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashe W. K., Notkins A. L. Neutralization of an infectious herpes simplex virus-antibody complex by anti-gamma-globulin. Proc Natl Acad Sci U S A. 1966 Aug;56(2):447–451. doi: 10.1073/pnas.56.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berry D. M., Almeida J. D. The morphological and biological effects of various antisera on avian infectious bronchitis virus. J Gen Virol. 1968 Jul;3(1):97–102. doi: 10.1099/0022-1317-3-1-97. [DOI] [PubMed] [Google Scholar]
  3. Daniels C. A., Borsos T., Rapp H. J., Snyderman R., Notkins A. L. Neutralization of sensitized virus by the fourth component of complement. Science. 1969 Aug 1;165(3892):508–509. doi: 10.1126/science.165.3892.508. [DOI] [PubMed] [Google Scholar]
  4. Hampar B., Notkins A. L., Mage M., Keehn M. A. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus. J Immunol. 1968 Mar;100(3):586–593. [PubMed] [Google Scholar]
  5. Heineman H. S. Herpes simplex neutralizing antibody--quantitation of the complement-dependent fraction in different phases of adult human infection. J Immunol. 1967 Jul;99(1):214–222. [PubMed] [Google Scholar]
  6. Jensen J. A. A specific inactivator of mammalian C'4 isolated from nurse shark (Ginglymostoma cirratum) serum. J Exp Med. 1969 Aug 1;130(2):217–241. doi: 10.1084/jem.130.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MUELLER-EBERHARD H. J., LEPOW I. H. C'1 ESTERASE EFFECT ON ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF THE FOURTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 May 1;121:819–833. doi: 10.1084/jem.121.5.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mardiney M. R., Jr, Müller-Eberhard H. J., Feldman J. D. Ultrastructural localization of the third and fourth components of complement on complement-cell complexes. Am J Pathol. 1968 Aug;53(2):253–261. [PMC free article] [PubMed] [Google Scholar]
  9. Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
  10. Notkins A. L., Mahar S., Scheele C., Goffman J. Infectious virus-antibody complex in the blood of chronically infected mice. J Exp Med. 1966 Jul 1;124(1):81–97. doi: 10.1084/jem.124.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rapp H. J., Borsos T. Complement research. Fundamental and applied. JAMA. 1966 Dec 26;198(13):1347–1354. [PubMed] [Google Scholar]
  12. Rother K., Rother U., Müller-Eberhard H. J., Nilsson J. R. Deficiency of the sixth component of complement in rabbits with an inherited complement defect. J Exp Med. 1966 Oct 1;124(4):773–785. doi: 10.1084/jem.124.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TANIGUCHI S., YOSHINO K. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. II. ANALYSIS OF COMPLEMENT AS THE ANTIBODY-POTENTIATING FACTOR. Virology. 1965 May;26:54–60. doi: 10.1016/0042-6822(65)90025-5. [DOI] [PubMed] [Google Scholar]
  14. TERRY W. D., BORSOS T., RAPP H. J. DIFFERENCES IN SERUM COMPLEMENT ACTIVITY AMONG INBRED STRAINS OF MICE. J Immunol. 1964 Apr;92:576–578. [PubMed] [Google Scholar]
  15. YOSHINO K., TANIGUCHI S. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. 3. MECHANISM OF THE ANTIBODY-POTENTIATING ACTION OF COMPLEMENT. Virology. 1965 May;26:61–72. doi: 10.1016/0042-6822(65)90026-7. [DOI] [PubMed] [Google Scholar]

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