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. 1970 Mar;65(3):625–632. doi: 10.1073/pnas.65.3.625

Derepression of GDP-α-D-Mannose and UDP-Glucose Pyrophosphorylases by a Regulator Gene Mutation; Episomal Dominance in Partial Diploids*

Michael M Lieberman 1,†, Christine E Buchanan 1, Alvin Markovitz 1
PMCID: PMC282952  PMID: 4910851

Abstract

Mutants of Escherichia coli K12 at the capR locus are overproduce capsular polysaccharide, and are derepressed for synthesis of several enzymes involved in capsular polysaccharide synthesis1-3 including GDP-mannose pyrophosphorylase.8 UDP-glucose pyrophosphorylase is also derepressed in a haploid capR9 mucoid mutant. Heterozygous mucoid partial diploids with the capR9 allele on the episome and the wild-type (capR+) allele on the chromosome (F′capR9/capR+) are derepressed for UDP-glucose pyrophosphorylase and GDP-mannose pyrophosphorylase, while the reciprocal nonmucoid heterozygotes (F′capR+/capR9) are repressed for these enzymes. These results provide evidence that the episomal capR allele is dominant with respect to synthesis of these two enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADLER H. I., HARDIGREE A. A. POSTIRRADIATION GROWTH, DIVISION, AND RECOVERY IN BACTERIA. Radiat Res. 1965 May;25:92–102. [PubMed] [Google Scholar]
  3. Alikhanian S. I., Pogosov V. Z. The fine structure of the gene controlling the synthesis of DNA polymerase in bacteriophage T4B. Genetics. 1969 Apr;61(4):773–776. doi: 10.1093/genetics/61.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernstein H., Fisher K. M. Dominance in bacteriophage T4D. Genetics. 1968 Mar;58(3):307–318. doi: 10.1093/genetics/58.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies J., Jacob F. Genetic mapping of the regulator and operator genes of the lac operon. J Mol Biol. 1968 Sep 28;36(3):413–417. doi: 10.1016/0022-2836(68)90165-4. [DOI] [PubMed] [Google Scholar]
  6. Donch J., Greenberg J. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol Gen Genet. 1968;103(2):105–115. doi: 10.1007/BF00427138. [DOI] [PubMed] [Google Scholar]
  7. Englesberg E., Squires C., Meronk F., Jr The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1100–1107. doi: 10.1073/pnas.62.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foley J. M., Giles N. H., Roberts C. F. Complementation at the adenylosuccinase locus in Aspergillus nidulans. Genetics. 1965 Dec;52(6):1247–1263. doi: 10.1093/genetics/52.6.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kalckar H. M., Kurahashi K., Jordan E. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776–1786. doi: 10.1073/pnas.45.12.1776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kang S., Markovitz A. Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase. J Bacteriol. 1967 Feb;93(2):584–591. doi: 10.1128/jb.93.2.584-591.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lieb M. Studies of heat-inducible lambda mutants. II. Production of C-1 product by superinfecting lambda+ in heat-inducible lysogens. Virology. 1966 Jul;29(3):367–376. doi: 10.1016/0042-6822(66)90212-1. [DOI] [PubMed] [Google Scholar]
  13. MARKOVITZ A. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1964 Feb;51:239–246. doi: 10.1073/pnas.51.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Markovitz A., Lieberman M. M., Rosenbaum N. Derepression of phosphomannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1497–1501. doi: 10.1128/jb.94.5.1497-1501.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Markovitz A., Rosenbaum N. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1084–1091. doi: 10.1073/pnas.54.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Markovitz A., Sydiskis R. J., Lieberman M. M. Genetic and biochemical studies on mannose-negative mutants that are deficient in phosphomannose isomerase in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1492–1496. doi: 10.1128/jb.94.5.1492-1496.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nikaido H., Levinthal M., Nikaido K., Nakane K. Extended deletions in the histidine-rough-B region of the Salmonella chromosome. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1825–1832. doi: 10.1073/pnas.57.6.1825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Uretz R. B., Markovitz A. Dominance of ultraviolet radiation resistance in partial diploids of Escherichia coli K-12. J Bacteriol. 1969 Nov;100(2):1118–1120. doi: 10.1128/jb.100.2.1118-1120.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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