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. 1970 Apr;65(4):865–871. doi: 10.1073/pnas.65.4.865

Proteins Specified by Herpes Simplex Virus, III. Viruses Differing in Their Effects on the Social Behavior of Infected Cells Specify Different Membrane Glycoproteins*

John M Keller 1,†, Patricia G Spear 1,‡, Bernard Roizman 1
PMCID: PMC282996  PMID: 4314902

Abstract

We examined the electrophoretic properties of the proteins and glycoproteins in the smooth membranes and virions purified from cells infected with herpes simplex virus strains differing with respect to their effects on the interaction of cells among themselves. The data show the following: (1) The glycoproteins in virions and binding to membranes share common features but vary quantitatively and qualitatively depending on the virus strain. (2) The binding to smooth membranes is ordered and not random. (3) Differences in the glycosylation of membrane proteins in African green monkey (VERO) and human (HEp-2) cells indicate that glycosylation is at least in part determined by the host.

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Selected References

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  1. Burge B. W., Strauss J. H., Jr Glycopeptides of the membrane glycoprotein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):449–466. doi: 10.1016/0022-2836(70)90314-1. [DOI] [PubMed] [Google Scholar]
  2. Burger M. M. A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc Natl Acad Sci U S A. 1969 Mar;62(3):994–1001. doi: 10.1073/pnas.62.3.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EPSTEIN M. A., ACHONG B. G., BARR Y. M. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Lancet. 1964 Mar 28;1(7335):702–703. doi: 10.1016/s0140-6736(64)91524-7. [DOI] [PubMed] [Google Scholar]
  4. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  5. HECHTER O. Reflections about hormone action and implications for the cancer problem. Cancer Res. 1957 Jun;17(5):512–519. [PubMed] [Google Scholar]
  6. Hakomori S. I., Murakami W. T. Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Jan;59(1):254–261. doi: 10.1073/pnas.59.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hakomori S. I., Teather C., Andrews H. Organizational difference of cell surface "hematoside" in normal and virally transformed cells. Biochem Biophys Res Commun. 1968 Nov 25;33(4):563–568. doi: 10.1016/0006-291x(68)90332-x. [DOI] [PubMed] [Google Scholar]
  8. Henle W., Henle G. Effect of arginine-deficient media on the herpes-type virus associated with cultured Burkitt tumor cells. J Virol. 1968 Mar;2(3):182–191. doi: 10.1128/jvi.2.3.182-191.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hsiung G. D., Kaplow L. S. Herpeslike virus isolated from spontaneously degenerated tissue culture derived from leukemia-susceptible guinea pigs. J Virol. 1969 Mar;3(3):355–357. doi: 10.1128/jvi.3.3.355-357.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meezan E., Wu H. C., Black P. H., Robbins P. W. Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography. Biochemistry. 1969 Jun;8(6):2518–2524. doi: 10.1021/bi00834a039. [DOI] [PubMed] [Google Scholar]
  12. Meléndez L. V., Hunt R. D., Daniel M. D., García F. G., Fraser C. E. Herpesvirus saimiri. II. Experimentally induced malignant lymphoma in primates. Lab Anim Care. 1969 Jun;19(3):378–386. [PubMed] [Google Scholar]
  13. Mora P. T., Brady R. O., Bradley R. M., McFarland V. W. Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1290–1296. doi: 10.1073/pnas.63.4.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pollack R. E., Burger M. M. Surface-specific characteristics of a contact-inhibited cell line containing the SV40 viral genome. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1074–1076. doi: 10.1073/pnas.62.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RAFFERTY K. A., Jr KIDNEY TUMORS OF THE LEOPARD FROG: A REVIEW. Cancer Res. 1964 Feb;24:169–185. [PubMed] [Google Scholar]
  16. ROIZMAN B. Polykaryocytosis. Cold Spring Harb Symp Quant Biol. 1962;27:327–342. doi: 10.1101/sqb.1962.027.001.031. [DOI] [PubMed] [Google Scholar]
  17. Schwartz J., Roizman B. Concerning the egress of herpes simplex virus from infected cells: electron and light microscope observations. Virology. 1969 May;38(1):42–49. doi: 10.1016/0042-6822(69)90126-3. [DOI] [PubMed] [Google Scholar]
  18. Schwartz J., Roizman B. Similarities and Differences in the Development of Laboratory Strains and Freshly Isolated Strains of Herpes Simplex Virus in HEp-2 Cells: Electron Microscopy. J Virol. 1969 Dec;4(6):879–889. doi: 10.1128/jvi.4.6.879-889.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Solomon J. J., Witter R. L., Nazerian K., Burmester B. R. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc Soc Exp Biol Med. 1968 Jan;127(1):173–177. doi: 10.3181/00379727-127-32649. [DOI] [PubMed] [Google Scholar]
  20. Spear P. G., Kellejmroian B. Proteins spcified by herpes simplex virus. II. Viral glycoprotins associated with cellular membranes. J Virol. 1970 Feb;5(2):123–131. doi: 10.1128/jvi.5.2.123-131.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Spear P. G., Roizman B. Buoyant density of herpes simplex virus in solutions of caesium chloride. Nature. 1967 May 13;214(5089):713–714. doi: 10.1038/214713a0. [DOI] [PubMed] [Google Scholar]
  22. Spear P. G., Roizman B. The proteins specified by herpes simplex virus. I. Time of synthesis, transfer into nuclei, and properties of proteins made in productively infected cells. Virology. 1968 Dec;36(4):545–555. doi: 10.1016/0042-6822(68)90186-4. [DOI] [PubMed] [Google Scholar]
  23. WILLMER E. N. Steroids and cell surfaces. Biol Rev Camb Philos Soc. 1961 Aug;36:368–398. doi: 10.1111/j.1469-185x.1961.tb01295.x. [DOI] [PubMed] [Google Scholar]
  24. Wallach D. F. Cellular membranes and tumor behavior: a new hypothesis. Proc Natl Acad Sci U S A. 1968 Nov;61(3):868–874. doi: 10.1073/pnas.61.3.868. [DOI] [PMC free article] [PubMed] [Google Scholar]

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