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. 1970 Apr;65(4):993–1000. doi: 10.1073/pnas.65.4.993

Immunochemical Measurement of Double-stranded RNA of Uninfected and Arbovirus-Infected Mammalian Cells*

Victor Stollar 1,2, B David Stollar 1,2
PMCID: PMC283014  PMID: 5266168

Abstract

A quantitative complement fixation assay which specifically measures double-stranded RNA has been used to study this RNA extracted from uninfected and arbovirus-infected cells. The double-straned RNA of the uninfected BHK-21 cells sedimented in the 12S region in sucrose gradients. The double-stranded RNA of Sinbis virus-infected cells, as measured immunochemically, included a predominant peak at 12S, a smaller 18S peak, and polydisperse material extending into the 26-30S region. All classes of this RNA detected immunochemically showed a sharp thermal denaturation curve, and increased in amount progressively during infection, with the 12S peak predominant at all times.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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